Three DNA Polymerases, Recruited by Different Mechanisms, Carry Out NER Repair Synthesis in Human Cells

被引：304

作者：

Ogi, Tomoo ^{[1
]}

Limsirichaikul, Siripan ^{[1
,2
]}

Overmeer, Rene M. ^{[3
]}

Volker, Marcel ^{[4
]}

Takenaka, Katsuya ^{[5
]}

Cloney, Ross ^{[4
]}

Nakazawa, Yuka ^{[1
]}

Niimi, Atsuko ^{[1
,4
]}

Miki, Yoshio ^{[5
]}

Jaspers, Nicolaas G. ^{[6
]}

Mullenders, Leon H. F. ^{[3
]}

Yamashita, Shunichi ^{[1
]}

Fousteri, Maria I. ^{[3
,7
]}

Lehmann, Alan R. ^{[4
]}

机构：

[1] Nagasaki Univ, Grad Sch Biomed Sci, Atom Bomb Dis Inst, Dept Mol Med, Nagasaki 8528523, Japan

[2] Silpakorn Univ, Fac Pharm, Dept Biopharm, Nakhon Pathom 73000, Thailand

[3] Leiden Univ, Med Ctr, Dept Toxicogenet, NL-2333 ZC Leiden, Netherlands

[4] Univ Sussex, Genome Damage & Stabil Ctr, Brighton BN1 9RQ, E Sussex, England

[5] Tokyo Med & Dent Univ, Med Res Inst, Dept Mol Genet, Bunkyo Ku, Tokyo 1138510, Japan

[6] Erasmus Univ, Med Ctr, Erasmus MC, Dept Genet, NL-3000 CA Rotterdam, Netherlands

[7] Biomed Sci Res Ctr Alexander Fleming, Vari 16672, Greece

来源：

MOLECULAR CELL | 2010年 / 37卷 / 05期

基金：

英国医学研究理事会; 日本科学技术振兴机构; 日本学术振兴会;

关键词：

NUCLEOTIDE EXCISION-REPAIR; TRANSLESION SYNTHESIS; Y-FAMILY; NUCLEAR ANTIGEN; HUMAN-FIBROBLASTS; 3RD SUBUNIT; POL-KAPPA; FACTOR-C; PCNA; REPLICATION;

D O I：

10.1016/j.molcel.2010.02.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleotide excision repair (NER) is the most versatile DNA repair system that deals with the major UV photoproducts in DNA, as well as many other DNA adducts. The early steps of NER are well understood, whereas the later steps of repair synthesis and ligation are not. In particular, which polymerases are definitely involved in repair synthesis and how they are recruited to the damaged sites has not yet been established. We report that, in human fibroblasts, approximately half of the repair synthesis requires both pol kappa and pol delta, and both polymerases can be recovered in the same repair complexes. Pol kappa is recruited to repair sites by ubiquitinated PCNA and XRCC1 and pol delta by the classical replication factor complex RFC1-RFC, together with a polymerase accessory factor, p66, and unmodified PCNA. The remaining repair synthesis is dependent on pol epsilon, recruitment of which is dependent on the alternative clamp loader CTF18-RFC.

引用

页码：714 / 727

页数：14

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