Versatile device for on-line and in-situ measurement of growth and light production of bioluminescent cells

被引:8
作者
Bendriaa, L
Picart, P
Daniel, P
Horry, H
Durand, MJ
Thouand, G
机构
[1] Ecole Natl Super Ingenieurs Mans, F-72085 Le Mans 9, France
[2] CNRS, UMR 6087, Lab Phys Etat Condense, F-72085 Le Mans, France
[3] IUT Genie Biol, BSCA, Lab CBAC, F-85000 La Roche Sur Yon, France
[4] Biolumine SA, Plateforme Vendee Rech, F-85600 Bouffere, France
关键词
opto-electronic bioreactor; bioluminescence; cell density; photomultiplier; photodiode; laser diode;
D O I
10.1016/j.snb.2004.04.043
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this paper, we present a new device composed of a biological part included in an original opto-electronic bioreactor described here in detail. It allows the simultaneous measurement of bioluminescence and optical density of a bioluminescent bacterial culture without any sampling. The cell density is measured through a laser diode and a photodiode by using a numerical synchronous detection and the bioluminescence with a photomultiplier. The experimental results are presented through the validation of the measurement principle using the Vibrio fischeri bacterium which was cultivated under continuous culture conditions. Very good correlation between manual and automatic measurements processed with the instrument has been demonstrated. The device could be applied to the realization of biosensors with any bioluminescent cells. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:115 / 121
页数:7
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