Comparative Analysis of the Expression of Glutathione Peroxidase and Glutathione Reductase Genes in Human Sperm after Cryopreservation

被引：14

作者：

Makarova, N. P. ^{[1
]}

Romanov, Yu. A. ^{[1
]}

Dolgushina, N. V. ^{[1
]}

Parker, M. M. ^{[1
]}

Krasnyi, A. M. ^{[1
]}

机构：

[1] Minist Hlth Russian Federat, VI Kulakov Natl Med Res Ctr Obstet Gynecol & Peri, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2018年 / 165卷 / 01期

关键词：

cryopreservation; oxidative stress; assisted reproductive technologies; sperm; cryotolerance; DNA-DAMAGE; SPERMATOZOA; CAPACITATION; HYPEROXIA;

D O I：

10.1007/s10517-018-4121-y

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We analyzed the effect of cryopreservation on the expression of glutathione peroxidase (GPX1) and glutathione reductase (GSR) genes in human sperm cells (15 sperm samples from fertile donors and 10 samples from infertile patients). The relative expression of GPX1 and GSR genes was determined by real-time PCR. The rate of post-thaw recovery was 2.1 times higher in the group of fertile donors. A significant increase in the expression of GPX1, but not GSR, was observed in sperm samples from infertile patients, while in patients with infertility, GPX1 expression significantly decreased after cryopreservation/thawing, in samples from fertile donors after the same procedure it increased to the level observed in the sperm samples from infertile patients. A positive correlation was revealed between GPX1 expression and sperm cryotolerance.

引用

页码：166 / 170

页数：5

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