Relationship between soluble intracellular endothelin-converting enzyme and endothelin-1 synthesis: Effect of inhibitors of the secretory pathway

被引:4
作者
Corder, R [1 ]
Khan, NQ [1 ]
Harrison, VJ [1 ]
Wood, EG [1 ]
Lees, DM [1 ]
Barker, S [1 ]
机构
[1] Univ London Queen Mary & Westfield Coll, St Bartholomews & Royal London Sch Med & Dent, William Harvey Res Inst, London EC1M 6BQ, England
关键词
endothelins (ET); endothelin; converting enzyme (ECE); endothelium; vasoconstrictor;
D O I
10.1097/00005344-200036051-00008
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The relationship between soluble and membrane-bound endothelin-converting; enzyme (ECE) activity with the level of endothelin-1 (ET-1) synthesis was investigated in cultured endothelial cells. Escherichia coli lipopolysaccharide (LPS) was used to stimulate ET-1 synthesis, and brefeldin A, monensin, colchicine or cytochalasin B, which disrupt peptide biosynthetic pathways in a variety of ways, were tested for their ability to modify changes in ET-1 synthesis and ECE levels. LPS increased ET-1 secretion by more than twofold. Levels of soluble ECE activity, but not those of membrane-bound ECE activity, correlated with ET-1 synthesis. These results suggest the soluble ECE activity is likely to play a role in ET-1 biosynthesis.
引用
收藏
页码:S19 / S21
页数:3
相关论文
共 12 条
  • [1] A SIMPLE METHOD FOR ISOLATING HUMAN ENDOTHELIN-CONVERTING ENZYME FREE FROM CONTAMINATION BY NEUTRAL ENDOPEPTIDASE-24.11
    CORDER, R
    KHAN, N
    HARRISON, VJ
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 207 (01) : 355 - 362
  • [2] The expression of endothelin-1 and endothelin-converting enzyme-1 (ECE-1) are independently regulated in bovine aortic endothelial cells
    Corder, R
    Barker, S
    [J]. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1999, 33 (04) : 671 - 677
  • [3] IDENTIFICATION OF ENDOTHELIN-1 AND BIG ENDOTHELIN-1 IN SECRETORY VESICLES ISOLATED FROM BOVINE AORTIC ENDOTHELIAL-CELLS
    HARRISON, VJ
    BARNES, K
    TURNER, AJ
    WOOD, E
    CORDER, R
    VANE, JR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (14) : 6344 - 6348
  • [4] ACTIN-DEPENDENT ACTIVATION OF ION CONDUCTANCES IN BRONCHIAL EPITHELIAL-CELLS
    HUG, T
    KOSLOWSKY, T
    ECKE, D
    GREGER, R
    KUNZELMANN, K
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1995, 429 (05): : 682 - 690
  • [5] DISRUPTION OF CYTOSKELETAL STRUCTURES MEDIATES SHEAR-STRESS INDUCED ENDOTHELIN-1 GENE-EXPRESSION IN CULTURED PORCINE AORTIC ENDOTHELIAL-CELLS
    MORITA, T
    KURIHARA, H
    MAEMURA, K
    YOSHIZUMI, M
    YAZAKI, Y
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (04) : 1706 - 1712
  • [6] Secretory pathways in endothelin synthesis
    Russell, FD
    Davenport, AP
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1999, 126 (02) : 391 - 398
  • [7] DIFFERING POLARITY OF THE CONSTITUTIVE AND REGULATED SECRETORY PATHWAYS FOR VONWILLEBRAND-FACTOR IN ENDOTHELIAL-CELLS
    SPORN, LA
    MARDER, VJ
    WAGNER, DD
    [J]. JOURNAL OF CELL BIOLOGY, 1989, 108 (04) : 1283 - 1289
  • [8] Isoforms of endothelin-converting enzyme: why and where?
    Turner, AJ
    Barnes, K
    Schweizer, A
    Valdenaire, O
    [J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 1998, 19 (12) : 483 - 486
  • [9] Regulation of parathyroid hormone-related protein secretion and mRNA expression in normal human keratinocytes and a squamous carcinoma cell line
    Weckmann, MT
    Grone, A
    Capen, CC
    Rosol, TJ
    [J]. EXPERIMENTAL CELL RESEARCH, 1997, 232 (01) : 79 - 89
  • [10] Woods M, 1999, MOL PHARMACOL, V55, P902