Incorporation of chitosan in biomimetic gelatin/chondroitin-6-sulfate/hyaluronan cryogel for cartilage tissue engineering

被引：91
作者：
Kuo, Chang-Yi [1 ]
Chen, Chih-Hao [1 ,2 ]
Hsiao, Chien-Yu [3 ]
Chen, Jyh-Ping [1 ,3 ]
机构：
[1] Chang Gung Univ, Dept Chem & Mat Engn, Taoyuan 333, Taiwan
[2] Chang Gung Univ, Chang Gung Mem Hosp, Dept Plast & Reconstruct Surg, Craniofacial Res Ctr,Coll Med, Taoyuan 333, Taiwan
[3] Chang Gung Univ Sci & Technol, Res Ctr Ind Human Ecol, Taoyuan 333, Taiwan
来源：
CARBOHYDRATE POLYMERS | 2015年 / 117卷
关键词：
Chitosan; Chondroitin sulfate; Hyaluronan; Cryogel; Cartilage tissue engineering; Gelatin; POLYMERIC CRYOGELS; PORE-SIZE; IN-VITRO; SCAFFOLD; CHONDROCYTES; HYDROGEL; COMPOSITES; MORPHOLOGY; CELLS; BONE;
D O I：
10.1016/j.carbpol.2014.10.056
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
We prepare an elastic macroporous gelatin/chondoitin-6-sulfate/hyaluronan (GCH) cryogel scaffold mimic the composition of cartilage extracellular matrix for cartilage tissue engineering. By incorporating chitosan in the cryogel to replace 20% gelatin, a GCH-chitosan cryogel was also synthesized and compared with GCH cryogel for scaffold mechanical properties and chondrocytes response. The GCH-chitosan cryogel has larger pores, higher ultimate strain (stress) and elastic modulus, and lower stress relaxation percentage than the GCH cryogel. Both cryogels show a highly elastic property with a loss tangent around 0.1, but chitosan incorporation increases the storage modulus (elasticity). Chondrocytes proliferate and redifferentiate in cryogels; chitosan diminishes cell proliferation but up-regulates glycosaminoglycans (GAGs) and type II collagen (COL II) secretion. Implantation of a chondrocytes/GCH-chitosan cryogel construct in a full-thickness articular cartilage defect regenerates cartilage with positive stainings for GAGs and COL II and an elastic modulus similar to the native cartilage. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：722 / 730
页数：9
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