EZH2 is involved in silencing of WNT5A during epithelial-mesenchymal transition of colon cancer cell line

被引:12
|
作者
Tao, Jianxin [1 ]
Shi, Liping [1 ]
Huang, Longchang [1 ]
Shi, Haoze [1 ]
Chen, Hang [1 ]
Wang, Yixin [1 ]
Wang, Tong [1 ]
机构
[1] Nanjing Med Univ, Dept Endoscopy Surg, Wuxi Peoples Hosp, Wuxi 214023, Peoples R China
关键词
Epithelial to mesenchymal transition; Transforming growth factor-beta; Enhancer of zeste Homolog 2; Histone; 3; lysine; 27; trimethylation; WNT signaling; HISTONE MODIFICATIONS; TUMOR-SUPPRESSOR; METHYLATION; METASTASIS; H3K27ME3; PROMOTER; METHYLTRANSFERASE; INHIBITION; CARCINOMA; GENISTEIN;
D O I
10.1007/s00432-017-2479-2
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Transforming growth factor-beta (TGF-beta) induction of epithelial-mesenchymal transition (EMT) in SW480 was established as a system for studies of colon cancer metastasis. However, the epigenetic mechanisms underlying this process remain unknown. In mammal, polycomb repressive complex-2 (PRC2) is a highly conserved histone methyltransferase involved in epigenetic regulations. Enhancer of zeste Homolog 2 (EZH2) is the catalytic subunit of PRC2, which catalyzes methylation of lysine 27 of histone H3 (H3K27). An inducible EMT system in colorectal cancer was utilized to study its mechanistic and phenotypic changes. Particularly, gene expression analysis was studied after immunoprecipitation. In this study, we reported that EZH2 is significantly enriched in the promoter region of WNT5A after TGF-beta induction in SW480 colon cancer cell line, which in turn silenced the expression of WNT5A. Furthermore, EZH2 inhibitor antagonized the TGF-beta-induced morphological conversion associated with epithelial-mesenchymal transition (EMT). Conversely, inhibition of histone H3K27me3 reader CBX does not affect the WNT5A expression level during TGF-beta-induced EMT. Our results indicate that EZH2 was essential for the silencing of WNT5A during TGF-beta-induced epithelial-mesenchymal transition of colon cancer cells.
引用
收藏
页码:2211 / 2219
页数:9
相关论文
共 50 条
  • [1] EZH2 is involved in silencing of WNT5A during epithelial–mesenchymal transition of colon cancer cell line
    Jianxin Tao
    Liping Shi
    Longchang Huang
    Haoze Shi
    Hang Chen
    Yixin Wang
    Tong Wang
    Journal of Cancer Research and Clinical Oncology, 2017, 143 : 2211 - 2219
  • [2] Wnt5a Suppresses Colon Cancer by Inhibiting Cell Proliferation and Epithelial-Mesenchymal Transition
    Cheng, Runfen
    Sun, Baocun
    Liu, Zhiyong
    Zhao, Xiulan
    Qi, Lisha
    Li, Yixian
    Gu, Qiang
    JOURNAL OF CELLULAR PHYSIOLOGY, 2014, 229 (12) : 1908 - 1917
  • [3] Chemomechanical regulation of EZH2 localization controls epithelial-mesenchymal transition
    Sacco, Jessica L.
    Vaneman, Zachary T.
    Self, Ava
    Sumner, Elix
    Kibinda, Stella
    Sankhe, Chinmay S.
    Gomez, Esther W.
    JOURNAL OF CELL SCIENCE, 2024, 137 (22)
  • [4] The Role and Significance of Wnt5a in Regulating Epithelial-Mesenchymal Transition in Endometrioid Adenocarcinoma
    Yang, Ningning
    Chen, Hongchun
    Huang, Yuchen
    Song, Xuexue
    Yang, Panpan
    Zhang, Shan
    Yan, Wentian
    Li, Nan
    Feng, Zhenzhong
    CANCER MANAGEMENT AND RESEARCH, 2021, 13 : 6527 - 6535
  • [5] Downregulation of EZH2 inhibits epithelial-mesenchymal transition in enzalutamide-resistant prostate cancer
    Zhang, Zhuangzhuang
    Wang, Xinyi
    Kim, Miyeong
    He, Daheng
    Wang, Chi
    Fong, Ka Wing
    Liu, Xiaoqi
    PROSTATE, 2023, 83 (15): : 1458 - 1469
  • [6] Epigenetic network of EZH2/SFRP1/Wnt in the epithelial-mesenchymal transition of laryngeal carcinoma cells
    Chen, Wei
    Wang, Hua-Tao
    Ji, Jun-Feng
    Wang, Zhi-Yi
    Shi, Tao
    Wu, Ming-Hai
    Yu, Peng-Ju
    NEOPLASMA, 2022, 69 (03) : 680 - 690
  • [7] WNT5A is a Key Regulator of the Epithelial-Mesenchymal Transition and Cancer Stem Cell Properties in Human Gastric Carcinoma Cells
    Kanzawa, Maki
    Semba, Shuho
    Hara, Shigeo
    Itoh, Tomoo
    Yokozaki, Hiroshi
    PATHOBIOLOGY, 2013, 80 (05) : 235 - 244
  • [8] WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition
    Chandra Prakash Prasad
    Shivendra Kumar Chaurasiya
    William Guilmain
    Tommy Andersson
    Journal of Experimental & Clinical Cancer Research, 35
  • [9] WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition
    Prasad, Chandra Prakash
    Chaurasiya, Shivendra Kumar
    Guilmain, William
    Andersson, Tommy
    JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2016, 35
  • [10] The Effect of EZH2 Inhibition through DZNep on Epithelial-Mesenchymal Transition Mechanism
    Ozel, Merve
    Kilic, Eser
    Baskol, Mevlut
    Akalin, Hilal
    Baskol, Gulden
    CELLULAR REPROGRAMMING, 2021, 23 (02) : 139 - 148