Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes

被引：6

作者：

Firouzamandi, Masoumeh ^{[1
,3
]}

Moeini, Hassan ^{[2
]}

Hosseini, Davood ^{[4
]}

Bejo, Mohd Hair ^{[3
]}

Omar, Abdul Rahman ^{[2
,3
]}

Mehrbod, Parvaneh ^{[3
]}

Ideris, Aini ^{[2
,3
]}

机构：

[1] Univ Tabriz, Fac Vet Med, Dept Pathobiol, Tabriz 5166616471, Iran

[2] Univ Putra Malaysia, Fac Vet Med, Inst Biosci, Serdang 43400, Malaysia

[3] Univ Putra Malaysia, Fac Vet Med, Dept Vet Clin Studies, Serdang 43400, Malaysia

[4] Razi Vaccine & Serum Res Inst, Arak 3197619751, Iran

来源：

JOURNAL OF VETERINARY SCIENCE | 2016年 / 17卷 / 01期

关键词：

DNA vaccine; Newcastle disease virus; antibody response; inactivated vaccine; PROTECTIVE IMMUNITY; CELLS; CHICKEN; NANOPARTICLES; IMMUNIZATION; VIRULENCE; VECTOR; NDV; HN;

D O I：

10.4142/jvs.2016.17.1.21

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant difference in enzyme-linked immunosorbent assay antibody levels (p < 0.05) elicited by either pIRES/F, pIRES/F+ pIRES/HN or pIRES-F/HN at one week after the booster in specific pathogen free chickens when compared with the inactivated vaccine alone. Taken together, these results indicated that recombinant pDNA could be used to increase the efficacy of the inactivated vaccine immunization procedure.

引用

页码：21 / 26

页数：6

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