Nucleosides and nucleotides.: 165.: Chemical ligation of oligodeoxynucleotides having a mercapto group at the 5-position of 2′-deoxyuridine via a disulfide bond

被引：6

作者：

Ueno, Y ^{[1
]}

Nakagawa, A ^{[1
]}

Matsuda, A ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Pharmaceut Sci, Kita Ku, Sapporo, Hokkaido 060, Japan

来源：

NUCLEOSIDES & NUCLEOTIDES | 1998年 / 17卷 / 1-3期

关键词：

D O I：

10.1080/07328319808005176

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the nonenzymatic ligation of oligodeoxynucleotides (ODNs) containing a mercapto group at the 5 position of 2'-deoxyuridine via a disulfide bond. Two ODNs containing different sequences were efficiently ligated in the presence of a template by this method.

引用

页码：283 / 289

页数：7

共 24 条

[11] SELECTIVE CHEMICAL AUTOLIGATION ON A DOUBLE-STRANDED DNA-TEMPLATE [J].

HERRLEIN, MK ;

LETSINGER, RL .

NUCLEIC ACIDS RESEARCH, 1994, 22 (23) :5076-5078

[12] RECOGNITION OF MIXED-SEQUENCE DUPLEX DNA BY ALTERNATE-STRAND TRIPLE-HELIX FORMATION [J].

HORNE, DA ;

DERVAN, PB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (06) :2435-2437

[13] MOLECULAR RECOGNITION BY CIRCULAR OLIGONUCLEOTIDES - INCREASING THE SELECTIVITY OF DNA-BINDING [J].

KOOL, ET .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (16) :6265-6266

[14] NONENZYMATIC SEQUENCE-SPECIFIC LIGATION OF DOUBLE-HELICAL DNA [J].

LUEBKE, KJ ;

DERVAN, PB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (19) :7447-7448

[15] NONENZYMATIC LIGATION OF DOUBLE-HELICAL DNA BY ALTERNATE-STRAND TRIPLE HELIX FORMATION [J].

LUEBKE, KJ ;

DERVAN, PB .

NUCLEIC ACIDS RESEARCH, 1992, 20 (12) :3005-3009

[16]

Maniatis T., 1982, MOL CLONING

[17]

NOMURA Y, IN PRESS NUCL ACIDS

[18] DNA TRIPLEX FORMATION OF OLIGONUCLEOTIDE ANALOGS CONSISTING OF LINKER GROUPS AND OCTAMER SEGMENTS THAT HAVE OPPOSITE SUGAR PHOSPHATE BACKBONE POLARITIES [J].

ONO, A ;

CHEN, CN ;

KAN, LS .

BIOCHEMISTRY, 1991, 30 (41) :9914-9921

[19] NUCLEOSIDES AND NUCLEOTIDES .127. A NOVEL AND CONVENIENT POST-SYNTHETIC MODIFICATION METHOD FOR THE SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES CARRYING AMINO LINKERS AT THE 5-POSITION OF 2'-DEOXYURIDINE [J].

ONO, A ;

HAGINOYA, N ;

KIYOKAWA, M ;

MINAKAWA, N ;

MATSUDA, A .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1994, 4 (02) :361-366

[20] STRUCTURAL EFFECTS IN THE RECOGNITION OF DNA BY CIRCULAR OLIGONUCLEOTIDES [J].

PRAKASH, G ;

KOOL, ET .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (09) :3523-3527

← 1 2 3 →