Plasmid-associated bacteriocin production by Lactobacillus LMG21688 suppresses Listeria monocytogenes growth rebound in a food system

被引:7
作者
Kouakou, Privat [1 ]
Dortu, Carine [1 ]
Dubois-Dauphin, Robin [1 ]
Vandenbol, Micheline [2 ]
Thonart, Philippe [1 ]
机构
[1] Fac Univ Sci Agron Gembloux, B-5030 Gembloux, Belgium
[2] Unite Biol Anim & Microbienne, Gembloux, Belgium
关键词
plasmid curing; PCR amplification; electrotransformation; Lactobacillus curvatus; bacteriocins; Listeria monocytogenes; LACTIC-ACID BACTERIA; ERYTHROMYCIN-RESISTANCE; CURVATUS CWBI-B28; MEAT; ELECTROPORATION; TRANSFORMATION; STRAIN; MILK;
D O I
10.1111/j.1574-6968.2010.01932.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacteriocin produced by Lactobacillus curvatus CWBI-B28wt is not completely effective against Listeria monocytogenes in food models. There is evidence suggesting that bacteriocin-degrading proteolytic enzymes produced by the CWBI-B28wt strain and/or present in the food matrix contribute to this rebound of Listeria growth. To limit this problem, we have partially characterized an approximately 10-kb plasmid responsible for bacteriocin production in L. curvatus CWBI-B28wt. This plasmid was transferred by high-voltage electroporation into a less proteolytic, but technologically competent Lactobacillus strain. When the transformed strain was used as a starter culture in a model food system, a high bacteriocin level was maintained for a longer time than with CWBI-B28wt, and Listeria growth rebound was delayed by 2 weeks (occurring after 3 weeks of apparently total inhibition, instead of one).
引用
收藏
页码:37 / 44
页数:8
相关论文
共 29 条
[1]   IDENTIFICATION AND CLONING OF A PLASMID-ENCODED ERYTHROMYCIN RESISTANCE DETERMINANT FROM LACTOBACILLUS-REUTERI [J].
AXELSSON, LT ;
AHRNE, SEI ;
ANDERSSON, MC ;
STAHL, SR .
PLASMID, 1988, 20 (02) :171-174
[2]   SPHEROPLAST AND ELECTROPORATION-MEDIATED TRANSFORMATION OF LACTOBACILLUS-PLANTARUM [J].
BADII, R ;
JONES, S ;
WARNER, PJ .
LETTERS IN APPLIED MICROBIOLOGY, 1989, 9 (02) :41-44
[3]   Biocontrol of Listeria monocytogenes in a model cultured milk (lben) by in situ bacteriocin production from Lactococcus lactis ssp lactis [J].
Benkerroum, N ;
Ghouati, Y ;
Ghalfi, H ;
Elmejdoub, T ;
Roblain, D ;
Jacques, P ;
Thonart, P .
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, 2002, 55 (03) :145-151
[4]  
CHASSY BM, 1987, FEMS MICROBIOL LETT, V46, P297, DOI 10.1111/j.1574-6968.1987.tb02467.x
[5]  
CHASSY BM, 1987, FEMS MICROBIOL LETT, V44, P173, DOI 10.1111/j.1574-6968.1987.tb02263.x
[6]   SPECTROPHOTOMETRIC ASSAY USING ORTHO-PHTHALDIALDEHYDE FOR DETERMINATION OF PROTEOLYSIS IN MILK AND ISOLATED MILK-PROTEINS [J].
CHURCH, FC ;
SWAISGOOD, HE ;
PORTER, DH ;
CATIGNANI, GL .
JOURNAL OF DAIRY SCIENCE, 1983, 66 (06) :1219-1227
[7]   FAILURE IN PRODUCTION OF CITRATE PERMEASE BY STREPTOCOCCUS DIACETILACTIS [J].
COLLINS, EB ;
HARVEY, RJ .
JOURNAL OF DAIRY SCIENCE, 1962, 45 (01) :32-&
[8]   Characterization of the tetracycline resistance plasmid pMD5057 from Lactobacillus plantarum 5057 reveals a composite structure [J].
Danielsen, M .
PLASMID, 2002, 48 (02) :98-103
[9]  
Diop MB, 2008, AFR J MICROBIOL RES, V2, P206
[10]   Anti-listerial activity of bacteriocin-producing Lactobacillus curvatus CWBI-B28 and Lactobacillus sakei CWBI-B1365 on raw beef and poultry meat [J].
Dortu, C. ;
Huch, M. ;
Holzapfel, W. H. ;
Franz, C. M. A. P. ;
Thonart, P. .
LETTERS IN APPLIED MICROBIOLOGY, 2008, 47 (06) :581-586