Verification and Unmasking of Widely Used Human Esophageal Adenocarcinoma Cell Lines

被引：102

作者：

Boonstra, Jurjen J. ^{[1
,2
]}

van Marion, Ronald ^{[1
,2
]}

Beer, David G. ^{[3
]}

Lin, Lin ^{[3
]}

Chaves, Paula ^{[4
]}

Ribeiro, Catarina ^{[4
]}

Pereira, A. Dias ^{[4
]}

Roque, Lucia ^{[4
]}

Darnton, S. Jane ^{[5
]}

Altorki, Nasser K. ^{[6
]}

Schrump, David S. ^{[7
]}

Klimstra, David S. ^{[8
]}

Tang, Laura H. ^{[8
]}

Eshleman, James R. ^{[9
]}

Alvarez, Hector ^{[9
]}

Shimada, Yutaka ^{[10
]}

van Dekken, Herman ^{[1
,2
]}

Tilanus, Hugo W.

Dinjens, Winand N. M. ^{[1
,2
]}

机构：

[1] Univ Med Ctr, Erasmus MC, Josephine Nefkens Inst, Dept Pathol, Rotterdam, Netherlands

[2] Univ Med Ctr, Erasmus MC, Josephine Nefkens Inst, Dept Surg, Rotterdam, Netherlands

[3] Univ Michigan, Dept Surg, Ann Arbor, MI 48109 USA

[4] IPOLFG, Grp Estudos Esofago Barrett, Lisbon, Portugal

[5] Birmingham Heartlands Hosp, Dept Thorac Surg, Esophageal Res Lab, Birmingham B9 5ST, W Midlands, England

[6] Cornell Univ, Coll Med, Dept Surg, New York, NY 10021 USA

[7] NCI, Thorac Oncol Sect, Surg Branch, Ctr Canc Res, Bethesda, MD 20892 USA

[8] Mem Sloan Kettering Canc Ctr, Dept Pathol, New York, NY 10021 USA

[9] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA

[10] Toyama Univ, Dept Surg, Toyama 930, Japan

来源：

JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE | 2010年 / 102卷 / 04期

关键词：

IN-VITRO; BARRETTS-ESOPHAGUS; ACID EXPOSURE; PROLIFERATION; MAPK; ESTABLISHMENT; APOPTOSIS; PATHWAYS; VIVO;

D O I：

10.1093/jnci/djp499

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the cell line. In a worldwide effort, we verified the authenticity of all available esophageal adenocarcinoma (EAC) cell lines. We proved that the frequently used cell lines SEG-1 and BIC-1 and the SK-GT-5 cell line are in fact cell lines from other tumor types. Experimental results based on these contaminated cell lines have led to ongoing clinical trials recruiting EAC patients, to more than 100 scientific publications, and to at least three National Institutes of Health cancer research grants and 11 US patents, which emphasizes the importance of our findings. Widespread use of contaminated cell lines threatens the development of treatment strategies for EAC.

引用

页码：271 / 274

页数：4

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