A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp

被引：12

作者：

Chimwai, Chaweewan ^{[1
]}

Tongboonsong, Punnee ^{[1
]}

Namramoon, Orathai ^{[1
]}

Panyim, Sakol ^{[1
,2
]}

Attasart, Pongsopee ^{[1
]}

机构：

[1] Mahidol Univ, Inst Mol Biosci, Salaya 73170, Nakhon Pathom, Thailand

[2] Mahidol Univ, Fac Sci, Dept Biochem, Bangkok 10400, Thailand

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2016年 / 134卷

关键词：

Penaeus monodon densovirus; Shrimp; RNAi; Double-stranded RNA; Oral delivery; PmDNV inhibition; PARVO-LIKE VIRUS; DISEASE;

D O I：

10.1016/j.jip.2016.01.003

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Penaeus monodon densovirus (PmDNV) is one of the major causes of stunted shrimp in the aquaculture industry in Thailand. Significant reductions in levels of PmDNV as assessed by PCR analysis of shrimp hepatopancreas were seen in both prophylactic and curative experiments after feeding shrimp with a formulated diet containing mixed inactivated bacteria harboring dsRNAs corresponding to the PmDNV ns1 and vp genes. Significant reductions of approximately 88% (prophylactic) and 64% (curative) of PmDNV were observed, suggesting that this diet has a high potential for application in commercial aquaculture for reducing PmDNV associated stunted growth of shrimp. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：23 / 26

页数：4

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