A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp

被引:12
作者
Chimwai, Chaweewan [1 ]
Tongboonsong, Punnee [1 ]
Namramoon, Orathai [1 ]
Panyim, Sakol [1 ,2 ]
Attasart, Pongsopee [1 ]
机构
[1] Mahidol Univ, Inst Mol Biosci, Salaya 73170, Nakhon Pathom, Thailand
[2] Mahidol Univ, Fac Sci, Dept Biochem, Bangkok 10400, Thailand
关键词
Penaeus monodon densovirus; Shrimp; RNAi; Double-stranded RNA; Oral delivery; PmDNV inhibition; PARVO-LIKE VIRUS; DISEASE;
D O I
10.1016/j.jip.2016.01.003
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Penaeus monodon densovirus (PmDNV) is one of the major causes of stunted shrimp in the aquaculture industry in Thailand. Significant reductions in levels of PmDNV as assessed by PCR analysis of shrimp hepatopancreas were seen in both prophylactic and curative experiments after feeding shrimp with a formulated diet containing mixed inactivated bacteria harboring dsRNAs corresponding to the PmDNV ns1 and vp genes. Significant reductions of approximately 88% (prophylactic) and 64% (curative) of PmDNV were observed, suggesting that this diet has a high potential for application in commercial aquaculture for reducing PmDNV associated stunted growth of shrimp. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码:23 / 26
页数:4
相关论文
共 14 条
[1]   Ingestion of bacteria expressing dsRNA triggers specific RNA silencing in shrimp [J].
Attasart, Pongsopee ;
Namramoon, Orathai ;
Kongphom, Ukrit ;
Chimwai, Chaweewan ;
Panyim, Sakol .
VIRUS RESEARCH, 2013, 171 (01) :252-256
[2]   Clearance of Penaeus monodon densovirus in naturally pre-infected shrimp by combined ns1 and vp dsRNAs [J].
Attasart, Pongsopee ;
Kaewkhaw, Rossukon ;
Chimwai, Chaweewan ;
Kongphom, Ukrit ;
Panyim, Sakol .
VIRUS RESEARCH, 2011, 159 (01) :79-82
[3]   Inhibition of Penaeus monodon densovirus replication in shrimp by double-stranded RNA [J].
Attasart, Pongsopee ;
Kaewkhaw, Rossukon ;
Chimwai, Chaweewan ;
Kongphom, Ukrit ;
Namramoon, Orathai ;
Panyim, Sakol .
ARCHIVES OF VIROLOGY, 2010, 155 (06) :825-832
[4]   CHARACTERIZATION OF HEPATOPANCREATIC PARVO-LIKE VIRUS, A 2ND UNUSUAL PARVOVIRUS PATHOGENIC FOR PENAEID SHRIMPS [J].
BONAMI, JR ;
MARI, J ;
POULOS, BT ;
LIGHTNER, DV .
JOURNAL OF GENERAL VIROLOGY, 1995, 76 :813-817
[5]  
Chalermporn Ongvarrasopone, 2007, ScienceAsia, V33, P35, DOI 10.2306/scienceasia1513-1874.2007.33.035
[6]   Statistical correlation between severity of hepatopancreatic parvovirus infection and stunting of farmed black tiger shrimp (Penaeus monodon) [J].
Flegel, TW ;
Thamavit, V ;
Pasharawipas, T ;
Alday-Sanz, V .
AQUACULTURE, 1999, 174 (3-4) :197-206
[7]   Protection of Macrobrachium rosenbergii against white tail disease by oral administration of bacterial expressed and encapsulated double-stranded RNA [J].
Kumar, Singaiah Naveen ;
Karunasagar, Indrani ;
Karunasagar, Iddya .
FISH & SHELLFISH IMMUNOLOGY, 2013, 35 (03) :833-839
[8]   A PARVO-LIKE VIRUS-DISEASE OF PENAEID SHRIMP [J].
LIGHTNER, DV ;
REDMAN, RM .
JOURNAL OF INVERTEBRATE PATHOLOGY, 1985, 45 (01) :47-53
[9]   Suppression of PmRab7 by dsRNA inhibits WSSV or YHV infection in shrimp [J].
Ongvarrasopone, Chalermporn ;
Chanasakulniyom, Mayuree ;
Sritunyalucksana, Kallaya ;
Panyim, Sakol .
MARINE BIOTECHNOLOGY, 2008, 10 (04) :374-381
[10]   Double-stranded RNA induces sequence-specific antiviral silencing in addition to nonspecific immunity in a marine shrimp: Convergence of RNA interference and innate immunity in the invertebrate antiviral response? [J].
Robalino, J ;
Bartlett, T ;
Shepard, E ;
Prior, S ;
Jaramillo, G ;
Scura, E ;
Chapman, RW ;
Gross, PS ;
Browdy, CL ;
Warr, GW .
JOURNAL OF VIROLOGY, 2005, 79 (21) :13561-13571