Design and validation of a clinical instrument for spectral diagnosis of cutaneous malignancy

被引：56

作者：

Rajaram, Narasimhan ^{[1
]}

Aramil, Timothy J. ^{[1
]}

Lee, Kelvin ^{[1
]}

Reichenberg, Jason S. ^{[2
]}

Nguyen, Tri H. ^{[3
]}

Tunnell, James W. ^{[1
]}

机构：

[1] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

[2] Univ Texas Med Branch, Dept Dermatol, Austin, TX 78701 USA

[3] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA

来源：

APPLIED OPTICS | 2010年 / 49卷 / 02期

基金：

美国国家卫生研究院;

关键词：

DIFFUSE-REFLECTANCE SPECTROSCOPY; LASER-INDUCED FLUORESCENCE; IN-VIVO MEASUREMENT; OPTICAL-PROPERTIES; MULTIEXCITATION FLUORESCENCE; INTRAEPITHELIAL NEOPLASIA; EXCITATION WAVELENGTHS; BARRETTS-ESOPHAGUS; LIGHT-SCATTERING; ANALYTICAL-MODEL;

D O I：

10.1364/AO.49.000142

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report a probe-based portable and clinically compatible instrument for the spectral diagnosis of melanoma and nonmelanoma skin cancers. The instrument combines two modalities-diffuse reflectance and intrinsic fluorescence spectroscopy-to provide complementary information regarding tissue morphology, function, and biochemical composition. The instrument provides a good signal-to-noise ratio for the collected reflectance and laser-induced fluorescence spectra. Validation experiments on tissue phantoms over a physiologically relevant range of albedos (0.35-0.99) demonstrate an accuracy of close to 10% in determining scattering, absorption and fluorescence characteristics. We also demonstrate the ability of our instrument to collect in vivo diffuse reflectance and fluorescence measurements from clinically normal skin, dysplastic nevus, and malignant nonmelanoma skin cancer. (C) 2010 Optical Society of America

引用

页码：142 / 152

页数：11

共 56 条

[1] In vivo measurement of the local optical properties of tissue by use of differential path-length spectroscopy [J].

Amelink, A ;

Sterenborg, HJCM ;

Bard, MPL ;

Burgers, SA .

OPTICS LETTERS, 2004, 29 (10) :1087-1089

[2]

[Anonymous], Cancer Facts and Figures 2009

[3] Diagnostics of pigmented skin tumors based on laser-induced autofluorescence and diffuse reflectance spectroscopy [J].

Borisova, E. ;

Troyanova, P. ;

Pavlova, P. ;

Avramov, L. .

QUANTUM ELECTRONICS, 2008, 38 (06) :597-605

[4] Fluorescence spectroscopy for in vivo characterization of ovarian tissue [J].

Brewer, M ;

Utzinger, U ;

Silva, E ;

Gershenson, D ;

Bast, RC ;

Follen, M ;

Richards-Kortum, R .

LASERS IN SURGERY AND MEDICINE, 2001, 29 (02) :128-135

[5] Combined reflectance and fluorescence spectroscopy for in vivo detection of cervical pre-cancer -: art. no. 024031 [J].

Chang, SK ;

Mirabal, YN ;

Atkinson, EN ;

Cox, D ;

Malpica, A ;

Follen, M ;

Richards-Kortum, R .

JOURNAL OF BIOMEDICAL OPTICS, 2005, 10 (02)

[6] Fluorescence spectroscopy of epithelial tissue throughout the dysplasia-carcinoma sequence in an animal model: Spectroscopic changes precede morphologic changes [J].

Coghlan, L ;

Utzinger, U ;

Richards-Kortum, R ;

Brookner, C ;

Zuluaga, A ;

Gimenez-Conti, I ;

Follen, M .

LASERS IN SURGERY AND MEDICINE, 2001, 29 (01) :1-10

[7] Optimal fluorescence excitation wavelengths for detection of squamous intra-epithelial neoplasia: results from an animal model [J].

Coghlan, L ;

Utzinger, U ;

Drezek, R ;

Heintzelman, D ;

Zuluaga, A ;

Brookner, C ;

Richards-Kortum, R ;

Gimenez-Conti, I ;

Follen, M .

OPTICS EXPRESS, 2000, 7 (12) :436-446

[8] Portable instrument that integrates irradiation with fluorescence and reflectance spectroscopies during clinical photodynamic therapy of cutaneous disease [J].

Cottrell, W. J. ;

Oseroff, A. R. ;

Foster, T. H. .

REVIEW OF SCIENTIFIC INSTRUMENTS, 2006, 77 (06)

[9]

Drezek R, 2001, PHOTOCHEM PHOTOBIOL, V73, P636, DOI 10.1562/0031-8655(2001)073<0636:AMOFCT>2.0.CO

[10]

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