Effects of Carbon Concentrations and Carbon to Nitrogen ratios on Sporulation of Two Biological Control Fungi as Determined by Different Culture Methods

The nematophagous fungus Pochonia chlamydosporia (Clavicipitaceae) and entomopathogenic fungus Beauveria bassiana (Cordycipitaceae) have great potential for biological control. However, a significant barrier to their commercial development as mycopesticides is the high costs associated with production. Carbon (C) concentration and C to nitrogen ratio (C:N ratio) greatly affect fungal growth and sporulation. Effects of C concentration and C:N ratio differed when the fungi were cultivated using two different methods:the conventional (continuous cultivation) method and a novel "two-stage" method. Sporulation of P. chlamydosporia (HSY-12-14) was the highest when the media contained 6 g l(-1) C and a C:N ratio of 40:1 or 8 g l(-1) C and C:N ratios of 20:1 or 40:1 for the conventional method but 8 g l(-1) C and a C:N ratio of only 10:1 with the novel "twostage" method. Sporulation of B. bassiana (IBC1201) was the highest when the media contained 12 g l(-1) C and a C:N ratio of 40:1 with the conventional method but only 4 g l(-1) C and a C:N ratio of 5:1 with the novel "two-stage" method. In addition, the nutritional requirements as determined by the conventional method differed for mycelial growth and sporulation. Understanding the effects of nutrition on sporulation can help programs seeking to use these organisms as biological control agents and is essential for their mass production and commercialization.