Liposome-based co-delivery of 7-O-geranyl-quercetin and IGF-1R siRNA for the synergistic treatment of non-small cell lung cancer

被引：15

作者：

Jiang, Meilin ^{[1
,2
]}

Zhang, Enxia ^{[3
]}

Liang, Ze ^{[3
]}

Zhao, Yinan ^{[4
]}

Zhang, Shubiao ^{[4
]}

Xu, Hong ^{[5
]}

Wang, Huaxin ^{[5
]}

Shu, Xiaohong ^{[3
]}

Kang, Xiaohui ^{[3
]}

Sun, Lidan ^{[1
]}

Zhen, Yuhong ^{[3
]}

机构：

[1] Dalian Med Univ, Affiliated Dalian Friendship Hosp, 8 Sanba Sq, Dalian 116001, Peoples R China

[2] Jinzhou Med Univ, Coll Postgrad, Jinzhou 121001, Peoples R China

[3] Dalian Med Univ, Coll Pharm, 9 Lvshun Southern Rd, Dalian 116044, Peoples R China

[4] Dalian Minzu Univ, Minist Educ, Key Lab Biotechnol & Bioresources Utilizat, Dalian 116600, Peoples R China

[5] Dalian Med Univ, Coll Basic Med Sci, Dalian 116044, Peoples R China

来源：

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY | 2019年 / 54卷

基金：

中国国家自然科学基金;

关键词：

7-O-Geranyl-quercetin; IGF-1R siRNA; Non-small cell lung cancer; Cationic liposome; Co-delivery; FACTOR-I RECEPTOR; SIGNALING PATHWAY; TUMOR-GROWTH; APOPTOSIS; 7-O-GERANYLQUERCETIN; OVEREXPRESSION; CHEMOTHERAPY; COMBINATION; INHIBITION; EXPRESSION;

D O I：

10.1016/j.jddst.2019.101316

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The combination of siRNAs and chemical drugs for the treatment of tumors has received more and more attention. This study investigated the synergistic effect of 7-O-geranyl quercetin (GQ) and IGF-1R siRNA (siIGF-1R) co-delivered by a liposome on human non-small cell lung cancer (NSCLC). GQ was firstly loaded with cationic liposome CDO14 to form CDO14-GQ which then combined with siIGF-1R to form co-delivery liposome CDO14-GQ-siIGF-1R. CCk-8 assay indicated that CDO14-GQ-siIGF-1R enhanced the anti-proliferation effect of CDO14-GQ or CDO14-siIGF-1R in NCI-H460 and A549 cells, AO/EB and AV/PI staining assays showed that the co-delivery liposome increased the pro-apoptosis effect of CDO14-GQ or CDO14-siIGF-1R. The growth inhibition effect of CDO14-GQ-siIGF-1R on the xenograft of NCI-H460 and A549 cells in mice was much stronger than that of CDO14-GQ or CDO14-siIGF-1R. Western blot assay indicated that CDO14-GQ-siIGF-1R down-regulated the expression levels of siIGF-1R in cells and in tumor tissues, and its effect on the expression of apoptosis-related proteins Bcl-2 and Bax was stronger than that of CDO14-GQ or CDO14-siIGF-1R. These results demonstrated that co-delivery of GQ and siIGF-1R by liposome improved the antitumor effect of either of them. Our study highlight that siRNAs combined with chemotherapeutic drugs is a promising strategy for the treatment of NSCLC.

引用

页数：8

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