Discovery and modulation of diterpenoid metabolism improves glandular trichome formation, artemisinin production and stress resilience in Artemisia annua

被引:23
作者
Chen, Ruibing [1 ]
Bu, Yuejuan [1 ]
Ren, Junze [1 ]
Pelot, Kyle A. [2 ]
Hu, Xiangyang [3 ]
Diao, Yong [4 ]
Chen, Wansheng [5 ,6 ]
Zerbe, Philipp [2 ]
Zhang, Lei [1 ,7 ]
机构
[1] Naval Med Univ, Sch Pharm, Dept Pharmaceut Bot, Shanghai 200433, Peoples R China
[2] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA
[3] Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China
[4] Huaqiao Univ, Sch Med, Quanzhou 362021, Peoples R China
[5] Naval Med Univ, Changzheng Hosp, Dept Pharm, Shanghai 200003, Peoples R China
[6] Shanghai Univ Tradit Chinese Med, Res & Dev Ctr Chinese Med Resources & Biotechnol, Shanghai 201203, Peoples R China
[7] Shanghai Univ, Biomed Innovat R&D Ctr, Sch Med, Shanghai 200444, Peoples R China
基金
中国国家自然科学基金;
关键词
Artemisia annua; artemisinin; diterpenoids; glandular trichome; stress resistance; COPALYL DIPHOSPHATE SYNTHASES; ENT-COPALYL; FUNCTIONAL-CHARACTERIZATION; GIBBERELLIN BIOSYNTHESIS; FUSARIUM-OXYSPORUM; MOLECULAR-CLONING; RICE; REDUCTASE; PATHWAYS; GENE;
D O I
10.1111/nph.17351
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plants synthesize diverse diterpenoids with numerous functions in organ development and stress resistance. However, the role of diterpenoids in glandular trichome (GT) development and GT-localized biosynthesis in plants remains unknown. Here, the identification of 10 diterpene synthases (diTPSs) revealed the diversity of diterpenoid biosynthesis in Artemisia annua. Protein-protein interactions (PPIs) between AaKSL1 and AaCPS2 in the plastids highlighted their potential functions in modulating metabolic flux to gibberellins (GAs) or ent-isopimara-7,15-diene-derived metabolites (IDMs) through metabolic engineering. A phenotypic analysis of transgenic plants suggested a complex repertoire of diterpenoids in Artemisia annua with important roles in GT formation, artemisinin accumulation and stress resilience. Metabolic engineering of diterpenoids simultaneously increased the artemisinin yield and stress resistance. Transcriptome and metabolic profiling suggested that bioactive GA(4)/GA(1) promote GT formation. Collectively, these results expand our knowledge of diterpenoids and show the potential of diterpenoids to simultaneously improve both the GT-localized metabolite yield and stress resistance, in planta.
引用
收藏
页码:2387 / 2403
页数:17
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