Effects of auxins and 2,3,5-triiodobenzoic acid on somatic embryo initiation from Norway spruce zygotic embryos (Picea abies)

被引:10
作者
Ramarosandratana, AV [1 ]
Van Staden, J [1 ]
机构
[1] Univ KwaZulu natal Pietermaritzburg, Res Ctr Plant Growth & Dev, ZA-3209 Scottsville, South Africa
基金
新加坡国家研究基金会;
关键词
conifer; polar auxin transport; somatic embryogenesis;
D O I
10.1023/B:TICU.0000049446.77837.d7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Embryonal-suspensormass (ESM) initiation from zygotic embryo sections was not affected by explant orientation whatever the type of external auxin used. Thus, polar auxin transport might not be directly involved in the control of somatic embryo formation. Application of 40 muM 2,3,5-triiodobenzoic acid ( TIBA) suppressed ESM initiation in hypocotyl sections. This effect of TIBA mimicked that of a supraoptimal dose of alpha-naphtaleneacetic acid suggesting a detrimental effect of fast internal auxin accumulation on ESM initiation.
引用
收藏
页码:105 / 107
页数:3
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