Preliminary study showing the relationship between platelet fibronectin, sialic acid, and ADP-induced aggregation levels in coronary heart disease

被引:3
作者
Butun, Ilknur I.
Ekmekci, Hakan
Sonmez, Huseyin
Gurel, Cigdem
Ciftci, Ozlem
Ulutin, Turgut
Kokoglu, Emine
Domanic, Nergiz
Dirican, Ahmet
机构
[1] Istanbul Univ, Cerrahpasa Med Sch, Dept Biochem, Istanbul, Turkey
[2] Istanbul Univ, Cerrahpasa Med Sch, Dept Paediat Haematol & Oncol, Bone Marrow Transplantat Unit, Istanbul, Turkey
[3] Istanbul Univ, Cerrahpasa Med Sch, Dept Med Biol, Istanbul, Turkey
[4] Istanbul Univ, Cerrahpasa Med Sch, Dept Cardiol, Istanbul, Turkey
[5] Istanbul Univ, Cerrahpasa Med Sch, Dept Biostat, Istanbul, Turkey
关键词
platelet aggregation; coronary heart disease; sialic acid; fibronectin;
D O I
10.1177/1076029607302434
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Several studies indicate that thrombosis plays an important role in the pathogenesis of coronary heart disease (CHD). Fibronectin is a multifunctional protein in plasma, other body fluids, and cell surface and plays an important role in platelet functions, including mediation of cell-cell and cell-surface interactions. Sialic acid is a regular constituent of glycoproteins and gangliozides in the outer cell membrane of mammalian cells. Therefore, the sialic acid content of platelets, which are characterized by their ability to aggregate with each other, can be important in leading to thrombus formation. In this study, platelet fibronectin, sialic acid-, and adenosine diphosphate (ADP)-induced platelet aggregation levels were determined in patients with CHD. Platelet sialic acid concentrations were determined by Warren's method. Platelet aggregation tests with ADP in platelet-rich plasma (PRP) were analyzed by use of an aggregometer. Platelet homogenate fibronectin levels were determined by ELISA. Total protein levels were determined by Lowry method. Our results indicate that, in patients with no vessel disease (patients with no obstructed vessel but suffering from chest pain, like angina pectoris) platelet fibronectin levels were significantly lower than the total of the other patients (patients with 1, 2, or 3 obstructed coronary vessels) (p < 0.05). Sialic acid levels in patients with no vessel disease were significantly lower than the total of the patient group (p < 0.05). There was significant (+) correlation between platelet aggregation, platelet fibronectin, platelet sialic acid, and severity of disease (p < 0.05). Our preliminary findings suggest that, especially platelet fibronectin levels potentially represent a pathogenic factor for CHD.
引用
收藏
页码:308 / 312
页数:5
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