Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes

被引：15

作者：

Larsen, Nicolai B. ^{[1
]}

Hickson, Ian D. ^{[1
]}

Mankouri, Hocine W. ^{[1
]}

机构：

[1] Univ Copenhagen, Dept Cellular & Mol Med, Ctr Healthy Aging, Copenhagen, Denmark

来源：

CELL CYCLE | 2014年 / 13卷 / 19期

基金：

欧洲研究理事会;

关键词：

DnaB helicase; replication fork; homologous recombination repair; RecQ helicase; MCM helicase; ESCHERICHIA-COLI; HOMOLOGOUS RECOMBINATION; MECHANISM; HELICASE; TERMINATION; PROTEIN; ARREST; FORKS; REARRANGEMENTS; PROGRESSION;

D O I：

10.4161/15384101.2014.958912

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.

引用

页码：2994 / 2998

页数：5

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