Functional characterization of Arabidopsis thaliana WRKY39 in heat stress

被引:233
作者
Li, Shujia [1 ,2 ]
Zhou, Xiang [2 ]
Chen, Ligang [2 ]
Huang, Weidong [1 ]
Yu, Diqiu [2 ]
机构
[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
[2] Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Kunming 650223, Peoples R China
基金
中国国家自然科学基金;
关键词
heat stress; jasmonate; salicylic acid; thermotolerance; WRKY39 transcription factor; SHOCK SIGNAL-TRANSDUCTION; DNA-BINDING PROTEINS; SALICYLIC-ACID; TRANSCRIPTION FACTORS; INDUCED THERMOTOLERANCE; BASAL THERMOTOLERANCE; ASCORBATE PEROXIDASE; ABSCISIC-ACID; DEFENSE; JASMONATE;
D O I
10.1007/s10059-010-0059-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arabidopsis thaliana WRKY39, a transcription factor that is induced by heat stress, is a member of the group II WRKY proteins and responds to both abiotic and biotic stress. Heat-treated seeds and plants of WRKY39 knock-down mutants had increased susceptibility to heat stress, showing reduced germination, decreased survival, and elevated electrolyte leakage compared with wild-type plants. In contrast, WRKY39 over-expressing plants exhibited enhanced thermotolerance compared with wild-type plants. RT-PCR and qRT-PCR analysis of wrky39 mutants and WRKY39 over-expressing plants identified putative genes regulated by WRKY39. Consistent with a role for WRKY39 in heat tolerance, the expression levels of salicylic acid (SA)-regulated PR1 and SA-related MBF1c genes were downregulated in wrky39 mutants. In contrast, over-expression of WRKY39 increased the expression of PR1 and MBF1c. The WRKY39 transcript was induced in response to treatment with SA or methyljasmonate. Analysis of heat stress-induced WRKY39 in defense signaling mutants, including coi1, ein2, and sid2, further indicated that WRKY39 was positively co-regulated by the SA and jasmonate (JA) signaling pathways. Together, these findings reveal that heat stress-induced WRKY39 positively regulates the cooperation between the SA- and JA-activated signaling pathways that mediate responses to heat stress.
引用
收藏
页码:475 / 483
页数:9
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