Non-invasive live-cell measurement of chancres in macrophage NAD(P)H by two-photon microscopy

被引：10

作者：

Kable, EPW

Kiemer, AK

机构：

[1] Univ Sydney, Australian Key Ctr Microscopy & Microanal, Electron Microscope Unit, Sydney, NSW 2006, Australia

[2] Univ Munich, Ctr Drug Res, Dept Pharm, D-81377 Munich, Germany

来源：

IMMUNOLOGY LETTERS | 2005年 / 96卷 / 01期

关键词：

NADPH oxidase; macrophages; reactive oxygen species; autofluorescence;

D O I：

10.1016/j.imlet.2003.12.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Two-photon microscopy allows determination of UV-excitable fluorophores using long-wavelength light. We aimed to determine NAD(P)H(P)H autofluorescence as a measure for macrophage NADPH-oxidase activation. RAW264.7 macrophages were grown on glass coverslips and kept in HBSS for microscopic investigation. Cells were excited with 710nm light and NAD(P)H autofluorescence was detected. Glucose as well as NaCN evoked an increase of NAD(P)H autofluorescence. Activators of NADPH oxidase lead to significantly decreased NAD(P)H autofluorescence. Therefore, this work shows the suitability of two-photon microscopy as a non-invasive method determining changes in phagocyte NAD(P)H upon activation. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：33 / 38

页数：6

共 3 条

[1] Non-Invasive Multi-Dimensional Two-Photon Microscopy enables optical fingerprinting (TPOF) of immune cells
Gehlsen, Uta
Szaszak, Marta
Gebert, Andreas
Koop, Norbert
Huettmann, Gereon
Steven, Philipp
JOURNAL OF BIOPHOTONICS, 2015, 8 (06) : 466 - 479
[2] Body regions have an impact on the collagen/elastin index of the skin measured by non-invasive in vivo vertical two-photon microscopy
Czekalla, Carolin
Schoenborn, Karl Heinz
Doege, Nadine
Jung, Sora
Darvin, Maxim E.
Lademann, Juergen
Meinke, Martina C.
EXPERIMENTAL DERMATOLOGY, 2017, 26 (09) : 822 - 824
[3] Formalin fixation and paraffin embedding interfere with the preservation of optical metabolic assessments based on endogenous NAD(P)H and FAD two-photon excited fluorescence
Sanchez-Hernandez, Adriana
Polleys, Christopher m.
Georgakoudi, Irene
BIOMEDICAL OPTICS EXPRESS, 2023, 14 (10) : 5238 - 5253

← 1 →