Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar

被引:14
作者
Tian, Yuru [1 ,2 ]
Li, Qianqian [1 ,2 ]
Rao, Shupei [1 ,2 ]
Wang, Aike [3 ,4 ]
Zhang, Hechen [5 ]
Wang, Liangsheng [6 ]
Li, Yue [1 ,2 ]
Chen, Jinhuan [1 ,2 ]
机构
[1] Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, 35 Qinghua East Rd, Beijing 100083, Peoples R China
[2] Beijing Forestry Univ, Natl Engn Lab Tree Breeding, 35 Qinghua East Rd, Beijing 100083, Peoples R China
[3] Yucheng Inst Agr Sci, Shangqiu 476000, Henan, Peoples R China
[4] Shangqiu Zhongxing Seedling Planting Co Ltd, Shangqiu 476000, Henan, Peoples R China
[5] Henan Acad Agr Sci, Hort Res Inst, Zhengzhou 450002, Henan, Peoples R China
[6] Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, 20 Nanxincun, Beijing 100093, Peoples R China
基金
中国国家自然科学基金;
关键词
anthocyanin; flavonoids; gene expression; leaf coloration; metabolic profiling; poplar; POPULUS; BIOSYNTHESIS; ACCUMULATION; TRANSCRIPTOME; EVOLUTION; ISOORIENTIN; REVEALS; COLORS; POTATO; MODEL;
D O I
10.1093/treephys/tpaa152
中图分类号
S7 [林业];
学科分类号
0829 ; 0907 ;
摘要
Poplar, a woody perennial model, is a common and widespread tree genus. We cultivated two red leaf poplar varieties from bud mutation of Populus sp. Linn. '2025' (also known as Zhonglin 2025, L2025 for shot): Populus deltoides varieties with bright red leaves (LHY) and completely red leaves (QHY). After measuring total contents of flavonoid, anthocyanin, chlorophyll and carotenoid metabolites, a liquid chromatography-electrospray ionization-tandem mass spectrometry system was used for the relative quantification of widely targeted metabolites in leaves of three poplar varieties. A total of 210 flavonoid metabolites (89 flavones, 40 flavonols, 25 flavanones, 18 anthocyanins, 16 isoflavones, 7 dihydroflavonols, 7 chalcones, 5 proanthocyanidins and 3 other flavonoid metabolites) were identified. Compared with L2025, 48 and 8 flavonoids were more and less abundant, respectively, in LHY, whereas 51 and 9 flavonoids were more and less abundant in QHY, respectively. On the basis of a comprehensive analysis of the metabolic network, gene expression levels were analyzed by deep sequencing to screen for potential reference genes for the red leaves. Most phenylpropanoid biosynthesis pathway-involved genes were differentially expressed among the examined varieties. Gene expression analysis also revealed several potential anthocyanin biosynthesis regulators including three MYB genes. The study results provide new insights into poplar flavonoid metabolites and represent the theoretical basis for future studies on leaf coloration in this model tree species.
引用
收藏
页码:1046 / 1064
页数:19
相关论文
共 50 条
  • [41] Gene expression and metabolite profiling analyses of developing pomegranate fruit peel reveal interactions between anthocyanin and punicalagin production
    Harel-Beja, Rotem
    Tian, Li
    Freilich, Shiri
    Habashi, Rida
    Borochov-Neori, Hamutal
    Lahav, Tamar
    Trainin, Taly
    Doron-Faigenboim, Adi
    Ophir, Ron
    Bar-Ya'akov, Irit
    Amir, Rachel
    Holland, Doron
    TREE GENETICS & GENOMES, 2019, 15 (02)
  • [42] Transcriptome profiling and differential gene expression analysis provides insights into Lr24-based resistance in wheat against Puccinia triticina
    Manjunatha Channappa
    Sapna Sharma
    Deepika Kulshreshtha
    Kartar Singh
    Subhash C. Bhardwaj
    Sivasamy Murugasamy
    A. Sindhu
    V. K. Vikas
    Rashmi Aggarwal
    3 Biotech, 2021, 11
  • [43] Transcriptome profiling and differential gene expression analysis provides insights into Lr24-based resistance in wheat against Puccinia triticina
    Channappa, Manjunatha
    Sharma, Sapna
    Kulshreshtha, Deepika
    Singh, Kartar
    Bhardwaj, Subhash C.
    Murugasamy, Sivasamy
    Sindhu, A.
    Vikas, V. K.
    Aggarwal, Rashmi
    3 BIOTECH, 2021, 11 (11)
  • [44] Poplar and Pathogen Interactions: Insights from Populus Genome-Wide Analyses of Resistance and Defense Gene Families and Gene Expression Profiling
    Duplessis, Sebastien
    Major, Ian
    Martin, Francis
    Seguin, Armand
    CRITICAL REVIEWS IN PLANT SCIENCES, 2009, 28 (05) : 309 - 334
  • [45] Transcriptomic and Metabolomic Profiling Provides Insights into Flavonoid Biosynthesis and Flower Coloring in Loropetalum chinense and Loropetalum chinense var. rubrum
    Zhang, Xia
    Zhang, Li
    Zhang, Damao
    Liu, Yang
    Lin, Ling
    Xiong, Xingyao
    Zhang, Donglin
    Sun, Ming
    Cai, Ming
    Yu, Xiaoying
    Li, Yanlin
    AGRONOMY-BASEL, 2023, 13 (05):
  • [46] EST-based Analysis of Gene Expression of Flavonoid Biosynthesis in Ginkgo Mature Leaves
    Wang, Yiqiang
    Ai, Binlin
    He, Xiaojing
    Tan, Xiaofeng
    Shen, Jikang
    Xing, Weiyi
    PROCEEDINGS OF 2009 INTERNATIONAL CONFERENCE OF NATURAL PRODUCT AND TRADITIONAL MEDICINE, VOLS 1 AND 2, 2009, : 411 - 416
  • [47] Combined transcriptome and proteome analysis provides insights into anthocyanin accumulation in the leaves of red-leaved poplars
    Xinghao Chen
    Hanqi Liu
    Shijie Wang
    Chao Zhang
    Lingyun Liu
    Minsheng Yang
    Jun Zhang
    Plant Molecular Biology, 2021, 106 : 491 - 503
  • [48] Global Analysis of Gene Expression Profiles Provides Novel Insights into the Development and Evolution of the Large Crustacean Eriocheir sinensis
    Wang, Jun
    Chen, Xiaowen
    He, Funan
    Song, Xiao
    Huang, Shu
    Yue, Wucheng
    Chen, Yipei
    Su, Zhixi
    Wang, Chenghui
    GENOMICS PROTEOMICS & BIOINFORMATICS, 2020, 18 (04) : 443 - 454
  • [49] Differential gene expression analysis of the resprouting process in Pinus canariensis provides new insights into a rare trait in conifers
    Chano, Victor
    Gailing, Oliver
    Collada, Carmen
    Soto, Alvaro
    PLANT GROWTH REGULATION, 2023, 100 (03) : 717 - 731
  • [50] Differential gene expression analysis of the resprouting process in Pinus canariensis provides new insights into a rare trait in conifers
    Víctor Chano
    Oliver Gailing
    Carmen Collada
    Álvaro Soto
    Plant Growth Regulation, 2023, 100 : 717 - 731