The Expression and Characterization of Highly Antigenic Region of Spike Protein of Prevalent Infectious Bronchitis Virus in Escherichia coli

被引：0
|
作者：
Zou, Nianli [1 ]
Zhao, Fangfang [1 ]
Liu, Ping [1 ]
Cao, Sanjie [1 ]
Wen, Xintian [1 ]
Huang, Yong [1 ,2 ]
机构：
[1] Sichuan Agr Univ, Coll Vet Med, Yaan 625014, Sichuan, Peoples R China
[2] Sichuan Agr Univ, Key Lab Anim Dis & Human Hlth Sichuan Prov, Yaan 625014, Sichuan, Peoples R China
来源：
JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2010年 / 9卷 / 08期
关键词：
Infectious Bronchitis Virus (IBV); Isolation; S1; gene; prokaryotic expression; pET-32a (+); HEMAGGLUTINATION-INHIBITION TEST; LINKED-IMMUNOSORBENT-ASSAY; MONOCLONAL-ANTIBODIES; MOLECULAR CHARACTERIZATION; STRUCTURAL PROTEINS; STRAINS; CHINA; CORONAVIRUSES; SEROTYPES; EPITOPES;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The serotypes and genotypes of Infectious Bronchitis Virus (IBV) was mainly determined by the Spike (S)1 glycoprotein. A prevalent IBV strain ZY3 was isolated and the highly antigenic region of its SI gene was amplified and expressed in Escherichia coli using the pET-32a (+) vector. The fusion protein, which was expressed at a high level was similar antigenically to the native S1 protein as determined by Western blot assay using rabbits polyclonal antibodies against IBV ZY3 strain. The fusion protein was also purified. This research lays the foundation for using this recombinant protein for development of indirect Enzyme-Linked Immunosorbent Assay (ELISA) for serum antibody detection or for production of monoclonal antibodies against prevalent IBV.
引用
收藏
页码：1267 / 1274
页数：8
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