Characterization of Aedes Dredd:: A novel initiator caspase from the yellow fever mosquito, Aedes aegypti

被引:24
|
作者
Cooper, Dawn M. [1 ]
Pio, Frederic
Thi, Emily P.
Theilmann, Dave
Lowenberger, Carl
机构
[1] Simon Fraser Univ, Dept Biol Sci, Burnaby, BC V5A 1S6, Canada
[2] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada
[3] Univ British Columbia, Agr & Agri Food Canada, Dept Agroecol, Summerland, BC, Canada
基金
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词
D O I
10.1016/j.ibmb.2007.03.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Caspases play an essential role during programmed cell death in all metazoans. These enzymes are cysteine proteases and comprise a multi-gene family with more than a dozen mammalian family members. Although caspases have been characterized in many animals, including Drosophila melanogaster, little is known about the laspases that exist in mosquitoes. Here we describe the identification and characterization of Aedes Dredd (AeDredd), a novel caspase in the yellow fever mosquito, Aedes aegypti. AeDredd contains two N-terminal death effector domains and the well conserved caspase catalytic domain. Multiple sequence alignments and functional substrate assays of recombinant protein Suggest that AcDredd is an orthologue of Drosophila Dredd and human caspase-8, both central effectors of the death receptor-inediated apoptotic pathway. AeDredd exhibits substrate specificity most similar to human caspase-8. AeDredd transcripts were found in all developmental stages with highest expression in early pupae. Within adults, AeDredd was found in all the tissues examined, with the highest transcript levels detected in fat body tissues. This is the first functional characterization of a death domain-containing caspase in in insect vector of human disease, and will initiate Studies on the role of apoptosis in the innate immune response of vectors towards intracellular parasites Such as Viruses. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:559 / 569
页数:11
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