Iron uptake in Ustilago maydis:: Tracking the iron path

被引:58
作者
Ardon, O
Nudelman, R
Caris, C
Libman, J
Shanzer, A
Chen, YN
Hadar, Y [1 ]
机构
[1] Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Dept Plant Pathol & Microbiol, IL-76100 Rehovot, Israel
[2] Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Otto Warburg Ctr Agr Biotechnol, IL-76100 Rehovot, Israel
[3] Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Dept Soil & Water Sci, IL-76100 Rehovot, Israel
[4] Weizmann Inst Sci, Dept Organ Chem, IL-76100 Rehovot, Israel
关键词
D O I
10.1128/JB.180.8.2021-2026.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In this study, we monitored and compared the uptake of iron in the fungus Ustilago maydis by using biomimetic siderophore analogs of ferrichrome, the fungal native siderophore, and ferrioxamine B (FOB), a xenosiderophore. Ferrichrome-iron was taken up at a higher rate than FOE-iron. Unlike ferrichrome-mediated uptake, FOE-mediated iron transport involved an extracellular reduction mechanism. By using fluorescently labeled siderophore analogs, we monitored the time course, as well as the localization, of iron uptake processes within the fungal cells. A fluorescently labeled ferrichrome analog, B9-lissamine rhodamine B, which does not exhibit fluorescence quenching upon iron binding, was used to monitor the entry of the compounds into the fungal cells. The fluorescence was found intracellularly 4 h after the application and later was found concentrated in two to three vesicles within each cell. The fluorescence of the fluorescently labeled FOE analog CAT18, which is quenched by iron, was visualized around the cell membrane after 4 h of incubation with the ferrated (nonfluorescent) compounds. This fluorescence intensity increased with time, demonstrating fungal iron uptake from the siderophores, which remained extracellular. We here introduce the use of fluorescent biomimetic siderophores as tools to directly track and discriminate between different pathways of iron uptake in cells.
引用
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页码:2021 / 2026
页数:6
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