Cutting Edge: CTNNBL1 Is Dispensable for Ig Class Switch Recombination

被引：22

作者：

Han, Li ^{[1
]}

Masani, Shahnaz ^{[1
]}

Yu, Kefei ^{[1
]}

机构：

[1] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA

来源：

JOURNAL OF IMMUNOLOGY | 2010年 / 185卷 / 03期

基金：

美国国家卫生研究院;

关键词：

CYTIDINE DEAMINASE AID; SOMATIC HYPERMUTATION; B-CELLS; ANTIBODY DIVERSIFICATION; GENE CONVERSION; DNA DEAMINATION; ENZYME; TRANSCRIPTS; EXPRESSION; MECHANISM;

D O I：

10.4049/jimmunol.1001643

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Ig class switch recombination (CSR) and somatic hypermutation require activation-induced cytidine deaminase (AID). The search for AID-interaction factors has been a major research effort in the field, as the mechanism of preferential targeting of AID to Ig loci remains elusive. CTNNBL1 is one of the few identified AID-interacting factors and has been shown to affect AID-mediated mutation and gene conversion in chicken DT40 cells. CTNNBL1 was also implicated in mammalian CSR by the fact that an AID mutant that fails to interact with CTNNBL1 also fails to support CSR in AID-deficient mouse B cells. To directly assess the role of CTNNBL1 in CSR, we disrupted the CTNNBL1 gene on both alleles in mouse CH12F3 cells by gene targeting. We found normal levels of CSR in CTNNBL1-deficient cells, indicating that CTNNBL1 is dispensable for CSR. The Journal of Immunology, 2010, 185: 1379-1381.

引用

页码：1379 / 1381

页数：3

共 23 条

[1] C-terminal deletion of AID uncouples class switch recombination from somatic hypermutation and gene conversion [J].

Barreto, V ;

Reina-San-Martin, B ;

Ramiro, AR ;

McBride, KM ;

Nussenzweig, MC .

MOLECULAR CELL, 2003, 12 (02) :501-508

[2] S-REGION TRANSCRIPTION PER-SE PROMOTES BASAL IGE CLASS SWITCH RECOMBINATION BUT ADDITIONAL FACTORS REGULATE THE EFFICIENCY OF THE PROCESS [J].

BOTTARO, A ;

LANSFORD, R ;

XU, LX ;

ZHANG, J ;

ROTHMAN, P ;

ALT, FW .

EMBO JOURNAL, 1994, 13 (03) :665-674

[3] Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase [J].

Bransteitter, R ;

Pham, P ;

Scharff, MD ;

Goodman, MF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :4102-4107

[4] Transcription-targeted DNA deamination by the AID antibody diversification enzyme [J].

Chaudhuri, J ;

Tian, M ;

Khuong, C ;

Chua, K ;

Pinaud, E ;

Alt, FW .

NATURE, 2003, 422 (6933) :726-730

[5] Interaction between anti body-diversification enzyme AID and spliceosome-associated factor CTNNBL1 [J].

Conticello, Silvestro G. ;

Ganesh, Karuna ;

Xue, Kanmin ;

Lu, Mason ;

Rada, Cristina ;

Neuberger, Michael S. .

MOLECULAR CELL, 2008, 31 (04) :474-484

[6] A coming-of-age story: activation-induced cytidine deaminase turns 10 [J].

Delker, Rebecca K. ;

Fugmann, Sebastian D. ;

Papavasiliou, F. Nina .

NATURE IMMUNOLOGY, 2009, 10 (11) :1147-1153

[7] Altering the pathway of immunoglobulin hypermutation by inhibiting uracil-DNA glycosylase [J].

Di Noia, J ;

Neuberger, MS .

NATURE, 2002, 419 (6902) :43-48

[8] Molecular Architecture of the Human Prp19/CDC5L Complex [J].

Grote, Michael ;

Wolf, Elmar ;

Will, Cindy L. ;

Lemm, Ira ;

Agafonov, Dmitry E. ;

Schomburg, Adrian ;

Fischle, Wolfgang ;

Urlaub, Henning ;

Luehrmann, Reinhard .

MOLECULAR AND CELLULAR BIOLOGY, 2010, 30 (09) :2105-2119

[9] Altered kinetics of nonhomologous end joining and class switch recombination in ligase IV-deficient B cells [J].

Han, Li ;

Yu, Kefei .

JOURNAL OF EXPERIMENTAL MEDICINE, 2008, 205 (12) :2745-2753

[10] Processing of switch transcripts is required for targeting of antibody class switch recombination [J].

Hein, K ;

Lorenz, MGO ;

Siebenkotten, G ;

Petry, K ;

Christine, R ;

Radbruch, A .

JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (12) :2369-2374

← 1 2 3 →