Evaluation of four commonly used normalizer genes for the study of decidual gene expression

被引：6

作者：

de Sousa, Ana Rita Sequeira ^{[1
,2
,3
,4
,5
]}

Staff, Anne Cathrine ^{[1
,2
,3
]}

Johnsen, Guro Mork ^{[1
,2
,3
,4
,5
]}

Weedon-Fekjaer, Mina Susanne ^{[1
,2
,3
]}

Storvold, Gro Leite ^{[1
,2
,3
,4
,5
]}

机构：

[1] Oslo Univ Hosp, Dept Obstet, Ulleval, Norway

[2] Oslo Univ Hosp, Dept Gynecol, Ulleval, Norway

[3] Univ Oslo, Fac Med, Oslo, Norway

[4] Oslo Univ Hosp, Expt Med Res Inst, Oslo, Norway

[5] Univ Oslo, Oslo, Norway

来源：

PLACENTA | 2016年 / 43卷

关键词：

Decidua basalis; Quantitative PCR; Gene expression; Preeclampsia; Normalizer gene; Housekeeping gene; HOUSEKEEPING GENES; PCR;

D O I：

10.1016/j.placenta.2016.04.009

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Reverse transcription quantitative PCR (RT-qPCR) gene expression results must be normalized using stably expressed genes to correct for technical variation. We evaluated the expression of four widely used normalizers (RNA18S, GAPDH, TBP, and YWHAZ) across 59 decidual tissue samples collected by vacuum suction from preeclamptic and normotensive pregnancies. RNA18S and GAPDH were not suitable as normalizers, while YWHAZ and TBP were stably expressed across the study groups. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：9 / 12

页数：4

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