Cloning and analysis of genomic differences unique to Burkholderia pseudomallei by comparison with B-thailandensis

被引：26

作者：

Brown, NF ^{[1
]}

Beacham, IR ^{[1
]}

机构：

[1] Griffith Univ, Sch Hlth Sci, Southport, Qld 4217, Australia

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2000年 / 49卷 / 11期

关键词：

D O I：

10.1099/0022-1317-49-11-993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Melioidosis is an infectious disease caused by Burkholderia pseudomallei. Genomic subtractive hybridisation was performed with the closely related avirulent species B, thailandensis to identify virulence genes of B, pseudomallei. The subtractive hybridisation products were highly specific for B, pseudomallei, Sequence analysis revealed a number of putative virulence factors, as well as apparently novel sequences of unknown function. The subtracted library contained DNA regions of relatively low G+C mol% content, which were distributed throughout the B, pseudomallei genome. The distribution of subtracted sequences amongst a collection of 22 B, pseudomallei isolates was found to be variable, with the exception of three strains which almost universally lacked the subtracted sequences. These three strains also differed in that they were highly haemolytic, indicating a possible separate virotype.

引用

页码：993 / 1001

页数：9

共 61 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] PRODUCTION OF HEMOLYSIN AND OTHER EXTRACELLULAR ENZYMES BY CLINICAL ISOLATES OF PSEUDOMONAS-PSEUDOMALLEI [J].

ASHDOWN, LR ;

KOEHLER, JM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (10) :2331-2334

[3]

Ausubel F. M., 1999, SHORT PROTOCOLS MOL

[4] CLONING, EXPRESSION, AND DNA-SEQUENCE ANALYSIS OF GENES ENCODING NONTYPABLE HAEMOPHILUS-INFLUENZAE HIGH-MOLECULAR-WEIGHT SURFACE-EXPOSED PROTEINS RELATED TO FILAMENTOUS HEMAGGLUTININ OF BORDETELLA-PERTUSSIS [J].

BARENKAMP, SJ ;

LEININGER, E .

INFECTION AND IMMUNITY, 1992, 60 (04) :1302-1313

[5] DIFFUSE ADHERENCE OF ENTEROPATHOGENIC ESCHERICHIA-COLI STRAINS [J].

BENZ, I ;

SCHMIDT, MA .

RESEARCH IN MICROBIOLOGY, 1990, 141 (7-8) :785-786

[6] ISOLATION AND SEROLOGIC CHARACTERIZATION OF AIDA-I, THE ADHESIN MEDIATING THE DIFFUSE ADHERENCE PHENOTYPE OF THE DIARRHEA-ASSOCIATED ESCHERICHIA-COLI STRAIN-2787 (O126-H27) [J].

BENZ, I ;

SCHMIDT, MA .

INFECTION AND IMMUNITY, 1992, 60 (01) :13-18

[7] Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei-like strains [J].

Brett, PJ ;

Deshazer, D ;

Woods, DE .

EPIDEMIOLOGY AND INFECTION, 1997, 118 (02) :137-148

[8] Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species [J].

Brett, PJ ;

DeShazer, D ;

Woods, DE .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :317-320

[9] Isolation and identification of Burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction [J].

Brook, MD ;

Currie, B ;

Desmarchelier, PM .

JOURNAL OF APPLIED MICROBIOLOGY, 1997, 82 (05) :589-596

[10] Identification of new transposable genetic elements in Burkholderia pseudomallei using subtractive hybridisation [J].

Brown, NF ;

Lew, AE ;

Beacham, IR .

FEMS MICROBIOLOGY LETTERS, 2000, 183 (01) :73-79

← 1 2 3 4 5 6 7 →