ESBL-producing E. coli in Austrian sewage sludge

被引:85
作者
Reinthaler, Franz Ferdinand [1 ]
Feierl, Gebhard [1 ]
Galler, Herbert [1 ]
Haas, Doris [1 ]
Leitner, Eva [1 ]
Mascher, Franz [1 ]
Melkes, Angelika [1 ]
Posch, Josefa [1 ]
Winter, Ingrid [2 ]
Zarfel, Gernot [1 ]
Marth, Egon [1 ]
机构
[1] Med Univ Graz, Inst Hyg Microbiol & Environm Med, A-8010 Graz, Austria
[2] Styrian Prov Govt, Specialized Div 19D, Waste & Mat Flow Management, Graz, Austria
关键词
Sewage sludge; Antibiotic resistance; E; coli; ESBL; SPECTRUM-BETA-LACTAMASE; ANTIBIOTIC-RESISTANCE; KLEBSIELLA-PNEUMONIAE; ESCHERICHIA-COLI; ENTEROBACTERIACEAE; INFECTION; SPREAD; FOOD;
D O I
10.1016/j.watres.2009.11.052
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The aim of this study was to investigate the degree of contamination of sewage sludge with ESBL-producing Escherichia coli strains and the effectiveness of different sewage sludge treatment methods. Monthly sewage sludge samples were collected between January and September 2009 in 5 different sewage treatment plants and tested for the presence of ESBL E. coli. in addition, the number of colony forming units (CFU) of E. coli and coliform, bacteria before and after the different sludge treatment methods (aerobic/anaerobic digestion, lime stabilization, and thermal treatment) was investigated. Of the 72 sewage sludge samples investigated, ESBL-positive E. coli were found in 44 (61.1%) sewage sludge samples. The classification of beta-lactamase groups was carried out in 15 strains resulting in the detection of 2 different groups (CTX-M and TEM) of bla genes. All 15 of them had a CTX-M gene and 4 of these strains furthermore carried a TEM gene. With regard to the CFU of E. coli and coliform bacteria, thermal treatment and lime stabilization following dehydration sufficiently reduced pathogen concentrations. The plants using merely stabilization and dehydration showed an increase of E. coli and coliform bacteria and thus also an increase in ESBL-producing E. coli. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1981 / 1985
页数:5
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