Differential ion dehydration energetics explains selectivity in the non-canonical lysosomal K+ channel TMEM175

被引:15
作者
Oh, SeCheol [1 ]
Marinelli, Fabrizio [2 ]
Zhou, Wenchang [2 ]
Lee, Jooyeon [3 ]
Choi, Ho Jeong [3 ]
Kim, Min [3 ]
Faraldo-Gomez, Jose D. [2 ]
Hite, Richard K. [1 ]
机构
[1] Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY 10065 USA
[2] NIH, Lung & Blood Inst, Theoret Mol Biophys Sect, Natl Heart, Bethesda, MD 20814 USA
[3] Chungbuk Natl Univ, Dept Chem, Cheongju, South Korea
来源
ELIFE | 2022年 / 11卷
关键词
ion channel; potassium channel; membrane transport; Human; FREE-ENERGY LANDSCAPES; BEAM-INDUCED MOTION; CRYO-EM STRUCTURE; MOLECULAR-DYNAMICS; POTASSIUM CHANNEL; FORCE-FIELD; CHARMM;
D O I
10.7554/eLife.75122
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Structures of the human lysosomal K+ channel transmembrane protein 175 (TMEM175) in open and closed states revealed a novel architecture lacking the canonical K+ selectivity filter motif present in previously known K+ channel structures. A hydrophobic constriction composed of four isoleucine residues was resolved in the pore and proposed to serve as the gate in the closed state, and to confer ion selectivity in the open state. Here, we achieve higher-resolution structures of the open and closed states and employ molecular dynamics simulations to analyze the conducting properties of the putative open state, demonstrating that it is permeable to K+ and, to a lesser degree, also Na+. Both cations must dehydrate significantly to penetrate the narrow hydrophobic constriction, but ion flow is assisted by a favorable electrostatic field generated by the protein that spans the length of the pore. The balance of these opposing energetic factors explains why permeation is feasible, and why TMEM175 is selective for K+ over Na+, despite the absence of the canonical selectivity filter. Accordingly, mutagenesis experiments reveal an exquisite sensitivity of the channel to perturbations that mitigate the constriction. Together, these data reveal a novel mechanism for selective permeation of ions by TMEM175 that is unlike that of other K+ channels.
引用
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页数:22
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