Oxidized Dextran Enhances Alternative Activation of Macrophages in Mice of Opposite Lines

被引：3

作者：

Chechushkov, A. V. ^{[1
]}

Kozhin, P. M. ^{[1
]}

Zaitseva, N. S. ^{[1
]}

Lemza, A. E. ^{[1
]}

Men'shchikova, E. B. ^{[1
]}

Troitskii, A. V. ^{[1
]}

Shkurupy, V. A. ^{[2
]}

机构：

[1] Res Inst Expt & Clin Med, Novosibirsk, Russia

[2] Novosibirsk State Med Univ, Minist Hlth Russian Federat, Novosibirsk, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2016年 / 160卷 / 06期

关键词：

60 kDa oxidized dextran; peritoneal macrophages; classical activation; alternative activation; C57Bl/6 and CBA mice; MYCOBACTERIUM-TUBERCULOSIS; MOUSE STRAINS; EXPRESSION; SYSTEM;

D O I：

10.1007/s10517-016-3309-2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Differences in peritoneal macrophage polarization in mice of opposite lines CBA and C57Bl/6 and the effects of 60 kDa oxidized dextran were studied. Macrophages of C57Bl/6 mice demonstrated a phenotype close to M1, with increasing expression of CD86 costimulatory molecule and unchanged CD206 expression in response to activation. Macrophages of CBA mice demonstrated higher plasticity in response to activating agents; expression of the markers increased irrespectively on stimulated receptor (TLR-4 or mannose receptor) and both CD86 (classical activation) and CD206 (alternative activation) increased. Macrophage response to addition of oxidized dextran (60 kDa) to the culture medium could be characterized as potentiation of their alternative activation: expression of CD86 in CBA mice in response to LPS and LPS+IL-4 and in C57Bl/6 mice in response to IFN-gamma and LPS+IFN-gamma decreased, while expression of CD206 by intact macrophages of CBA mice and by macrophages stimulated by IFN-gamma and IL-4 increased under the effect of 60 kDa oxidized dextran.

引用

页码：783 / 786

页数：4

共 15 条

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