Small interfering RNA targeting CD81 ameliorated arthritis in rats

被引：23
|
作者：
Nakagawa, Shuji [2 ]
Arai, Yuji [2 ]
Mori, Hiroki [1 ]
Matsushita, Yumi [3 ]
Kubo, Toshikazu [2 ]
Nakanishi, Tohru [1 ]
机构：
[1] Shujitsu Univ, Sch Pharm, Okayama 7038516, Japan
[2] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Orthopaed, Kamigyo Ku, Kyoto 6028566, Japan
[3] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Oral Pathol, Okayama 7008525, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 388卷 / 03期
关键词：
Arthritis; siRNA; CD81; Synoviolin; Collagen-induced arthritic rat; Synovial tissue; Intraarticular injection; In vivo electroporation; NECROSIS-FACTOR-ALPHA; RHEUMATOID-ARTHRITIS; MONOCLONAL-ANTIBODY; SIGNAL-TRANSDUCTION; INFLIXIMAB; PLACEBO; GENES; CELLS; TRIAL;
D O I：
10.1016/j.bbrc.2009.06.150
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
CD81 belongs to a family of cell-surface protein ( tetraspanin) known as one of the up-regulated elements in rheumatoid arthritis synoviocytes. In this study, the therapeutic effect of small interfering RNA targeting CD81 (siCD81) was examined by in vivo electroporation method. Treatment with siCD81 significantly ameliorated paw swelling of collagen-induced arthritic ( CIA) rats. In histological examination, hypertrophy of synovium, bone erosion, and degeneration of articular cartilage were milder in rats treated with siCD81 than in the control group and the non-specific siRNA group. Expression of synoviolin, a rheumatoid regulator, was suppressed by siCD81. Thus, therapeutic intervention by targeting CD81 may be used in the treatment of rheumatoid arthritis. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：467 / 472
页数：6
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