Microwave, ultrasound, thermal treatments, and bead milling as intensification techniques for extraction of lipids from oleaginous Yarrowia lipolytica yeast for a biojetfuel application

被引:138
作者
Meullemiestre, Alice [1 ]
Breil, Cassandra [1 ]
Abert-Vian, Maryline [1 ]
Chemat, Farid [1 ]
机构
[1] Univ Avignon & Pays Vaucluse, INRA, UMR 408, GREEN Extract Team, F-84000 Avignon, France
关键词
Lipid extraction; Microwave; Ultrasound; Bead milling; Oleaginous microorganisms; FATTY-ACID; MICROALGAE; OIL; OPTIMIZATION; PURIFICATION; DEGRADATION; LEAVES;
D O I
10.1016/j.biortech.2016.03.040
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
In the present work, two different ways of lipids extraction from Yarrowia lipolytica yeast were investigated in order to maximize the extraction yield. Firstly, various modern techniques of extraction including ultrasound, microwave, and bead milling were tested to intensify the efficiency of lipid recovery. Secondly, several pretreatments such as freezing/defrosting, cold drying, bead milling, and microwave prior two washing of mixture solvent of chloroform: methanol (1: 2, v/v) were study to evaluate the impact on lipid recovery. All these treatments were compared to conventional maceration, in terms of lipids extraction yield and lipid composition analysis. The main result of this study is the large difference of lipid recovery among treatments and the alteration of lipids profile after microwave and ultrasound techniques. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:190 / 199
页数:10
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