Neutrophil Expression of Cyclooxygenase 2 in Preeclampsia

被引：16
作者：
Bachawaty, Teddi [1 ,2 ]
Washington, Sonya L. [1 ,2 ]
Walsh, Scott W. [1 ,2 ]
机构：
[1] Virginia Commonwealth Univ, Med Ctr, Dept Obstet & Gynecol, Richmond, VA 23298 USA
[2] Virginia Commonwealth Univ, Med Ctr, Dept Physiol & Biophys, Richmond, VA 23298 USA
来源：
REPRODUCTIVE SCIENCES | 2010年 / 17卷 / 05期
基金：
美国国家卫生研究院;
关键词：
Preeclampsia; pregnancy; neutrophils; COX-2; interleukin; 8; ARACHIDONIC-ACID; METABOLIC CHARACTERISTICS; NORMAL-PREGNANCY; LINOLEIC-ACID; WOMEN; THROMBOXANE; MONOCYTES; INTERLEUKIN-8; GRANULOCYTES; ACTIVATION;
D O I：
10.1177/1933719110361960
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
The objective of this study was to determine whether (1) neutrophils of preeclamptic women express cyclooxygenase 2 (COX-2) and (2) COX-2 mediates neutrophil interleukin 8 (IL-8) production. Neutrophils were isolated from heparinized blood collected from women with preeclampsia and normal pregnancy and from normal nonpregnant women. Cyclooxygenase 2 protein was measured in the neutrophils of the 3 groups by specific enzyme-linked immunosorbent assay (ELISA). To study the role of COX-2 in IL-8 production, neutrophils of normal pregnant women were activated with arachidonic acid (AA) and incubated overnight in Medium 199 with and without NS398, a specific COX-2 inhibitor. We found that neutrophils from preeclamptic women expressed significantly more COX-2 than neutrophils from normal pregnant or normal nonpregnant women. Arachidonic acid activated neutrophils to produce IL-8, which was completely inhibited by NS398. These data demonstrate that COX-2 is expressed in neutrophils of preeclamptic women and that COX-2 mediates IL-8 production by activated neutrophils.
引用
收藏
页码：465 / 470
页数：6
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