Generation of infectious and pathogenic duck hepatitis virus type 1 from cloned full-length cDNA

被引：10
|
作者：
Yun, T. [2 ,3 ]
Ni, Z. [3 ]
Liu, G. Q. [1 ]
Yu, B. [3 ]
Chen, L. [3 ]
Huang, J. G. [3 ]
Zhang, Y. M. [2 ]
Chen, J. P. [3 ]
机构：
[1] Chinese Acad Agr Sci, Dept Avian Infect Dis, Shanghai Vet Res Inst, Key Lab Anim Parasitol,Chines Minist Agr, Shanghai 200241, Peoples R China
[2] NW A&F Univ, Coll Vet Med, Yongling 712100, Shaanxi, Peoples R China
[3] Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China
来源：
VIRUS RESEARCH | 2010年 / 147卷 / 02期
关键词：
RNA viruses; Picornaviruses; Infectious cDNA; Duck hepatitis virus type 1; MOLECULAR ANALYSIS; NUCLEOTIDE-SEQUENCE; TYROSINE RESIDUE; VIRAL-RNA; PROTEIN; VPG; TRANSCRIPTS; POLYMERASE; POLIOVIRUS; DUCKLINGS;
D O I：
10.1016/j.virusres.2009.10.014
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A full-length cDNA clone of duck hepatitis virus type 1 (DHV-1) isolated from duck was assembled in the plasmid vector pBluescript II SK+. RNA synthesized in vitro by means of a Sp6 promoter inserted in front of the cDNA produced infectious particles after transfection of BHK-21 cells, as shown by the appearance of cytopathic effect. The rescued virus was also found to be highly pathogenic to young ducks by intradermal injection and resulted in a fatal disease indistinguishable from that of wild-type virus. The availability of the first cDNA clone of DHV-1 will allow examination of the molecular mechanisms behind DHV-1 virulence and attenuation, which could in turn lead to the production of second-generation, genetically engineered DHV-1 vaccines. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：159 / 165
页数：7
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