Generation of infectious and pathogenic duck hepatitis virus type 1 from cloned full-length cDNA

被引：10

作者：

Yun, T. ^{[2
,3
]}

Ni, Z. ^{[3
]}

Liu, G. Q. ^{[1
]}

Yu, B. ^{[3
]}

Chen, L. ^{[3
]}

Huang, J. G. ^{[3
]}

Zhang, Y. M. ^{[2
]}

Chen, J. P. ^{[3
]}

机构：

[1] Chinese Acad Agr Sci, Dept Avian Infect Dis, Shanghai Vet Res Inst, Key Lab Anim Parasitol,Chines Minist Agr, Shanghai 200241, Peoples R China

[2] NW A&F Univ, Coll Vet Med, Yongling 712100, Shaanxi, Peoples R China

[3] Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China

来源：

VIRUS RESEARCH | 2010年 / 147卷 / 02期

关键词：

RNA viruses; Picornaviruses; Infectious cDNA; Duck hepatitis virus type 1; MOLECULAR ANALYSIS; NUCLEOTIDE-SEQUENCE; TYROSINE RESIDUE; VIRAL-RNA; PROTEIN; VPG; TRANSCRIPTS; POLYMERASE; POLIOVIRUS; DUCKLINGS;

D O I：

10.1016/j.virusres.2009.10.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A full-length cDNA clone of duck hepatitis virus type 1 (DHV-1) isolated from duck was assembled in the plasmid vector pBluescript II SK+. RNA synthesized in vitro by means of a Sp6 promoter inserted in front of the cDNA produced infectious particles after transfection of BHK-21 cells, as shown by the appearance of cytopathic effect. The rescued virus was also found to be highly pathogenic to young ducks by intradermal injection and resulted in a fatal disease indistinguishable from that of wild-type virus. The availability of the first cDNA clone of DHV-1 will allow examination of the molecular mechanisms behind DHV-1 virulence and attenuation, which could in turn lead to the production of second-generation, genetically engineered DHV-1 vaccines. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：159 / 165

页数：7

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