Insecticide susceptibility of natural populations of Anopheles coluzzii and Anopheles gambiae (sensu stricto) from Okyereko irrigation site, Ghana, West Africa

被引：20

作者：

Chabi, Joseph ^{[1
,2
]}

Baidoo, Philip K. ^{[3
]}

Datsomor, Alex K. ^{[1
,2
]}

Okyere, Dora ^{[1
,2
]}

Ablorde, Aikins ^{[1
,2
]}

Iddrisu, Alidu ^{[1
,2
]}

Wilson, Michael D. ^{[2
]}

Dadzie, Samuel K. ^{[2
]}

Jamet, Helen P. ^{[4
]}

Diclaro, Joseph W., II ^{[5
]}

机构：

[1] Univ Ghana, Noguchi Mem Inst Med Res, Vestergaard NMIMR Vector Labs VNVL, Legon, Accra, Ghana

[2] Univ Ghana, Noguchi Mem Inst Med Res, Dept Parasitol, Legon, Accra, Ghana

[3] Kwame Nkrumah Univ Sci & Technol, Dept Theoret & Appl Biol, Kumasi, Ghana

[4] Vestergaard, Washington, DC USA

[5] US Naval Med Res Unit 3, Vector Biol Res Program, Cairo, Egypt

来源：

PARASITES & VECTORS | 2016年 / 9卷

关键词：

Insecticide resistance; Malaria; An. gambiae s.s; An; coluzzii; Irrigation; KNOCKDOWN RESISTANCE MUTATION; MAJOR MALARIA VECTOR; RICE-GROWING AREA; P-GLYCOPROTEIN; TREATED NETS; MOLECULAR-FORMS; BURKINA-FASO; GENETIC DIFFERENTIATION; PYRETHROID RESISTANCE; BORNE DISEASES;

D O I：

10.1186/s13071-016-1462-0

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background: The increasing spread of insecticide resistance in malaria vectors has been well documented across sub-Saharan Africa countries. The influence of irrigation on increasing vector resistance is poorly understood, and is critical to successful and ethical implementation of food security policies. This study investigated the insecticide resistance status of An. gambiae (s.l.) mosquitoes collected from the irrigated rice area of Okyereko, a village containing about 42 hectares of irrigated field within an irrigation project plan in the Central Region of Ghana. Large amounts of insecticides, herbicides and fertilizers are commonly used in the area to boost the annual production of the rice. Methods: Mosquito larvae were collected and adults were assayed from the F-1 progeny. The resistance status, allele and genotype were characterized using WHO susceptibility testing and PCR methods respectively. Results: The An. gambiae (s.l.) populations from Okyereko are highly resistant to DDT and pyrethroid insecticides, with possible involvement of metabolic mechanisms including the elevation of P450 and GST enzyme as well as P-gp activity. The population was mostly composed of An. coluzzii specimens (more than 96 %) with kdr and ace-1 frequencies of 0.9 and 0.2 %, respectively. Conclusion: This study brings additional information on insecticide resistance and the characterization of An. gambiae (s.l.) mosquitoes from Okyereko, which can be helpful in decision making for vector control programmes in the region.

引用

页数：8

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