A Comprehensive Phytochemical Analysis of Terpenes, Polyphenols and Cannabinoids, and Micromorphological Characterization of 9 Commercial Varieties of Cannabis sativa L.

被引：29

作者：

Mazzara, Eugenia ^{[1
]}

Torresi, Jacopo ^{[1
]}

Fico, Gelsomina ^{[2
,3
]}

Papini, Alessio ^{[4
]}

Kulbaka, Nicola ^{[5
]}

Dall'Acqua, Stefano ^{[6
]}

Sut, Stefania ^{[6
]}

Garzoli, Stefania ^{[7
]}

Mustafa, Ahmed M. ^{[1
,8
]}

Cappellacci, Loredana ^{[1
]}

Fiorini, Dennis ^{[9
]}

Maggi, Filippo ^{[1
]}

Giuliani, Claudia ^{[2
,3
]}

Petrelli, Riccardo ^{[1
]}

机构：

[1] Univ Camerino, Sch Pharm, Chem Interdisciplinary Project CHIP, Via Madonna Carceri, I-62032 Camerino, Italy

[2] Univ Milan, Dept Pharmaceut Sci, Via Mangiagalli 25, I-20133 Milan, Italy

[3] Univ Milan, Dept Pharmaceut Sci, Ghirardi Bot Garden, Via Relig 25, I-25088 Toscolano Maderno, Italy

[4] Univ Florence, Dept Biol, Via Pira 4, I-50121 Florence, Italy

[5] Soc Agr Everweed G Di Vietri & C SS, Frazione Conti 2, I-63857 Amandola, Italy

[6] Univ Padua, Dept Pharmaceut & Pharmacol Sci, Nat Prod Lab, I-35131 Padua, Italy

[7] Sapienza Univ, Dept Drug Chem & Technol, I-00185 Rome, Italy

[8] Zagazig Univ, Fac Pharm, Dept Pharmacognosy, Zagazig 44519, Egypt

[9] Univ Camerino, Sch Sci & Technol, CHEM INTERDISCIPLINARY PROJECT CHIP, Via Madonna Carceri, I-62032 Camerino, Italy

来源：

PLANTS-BASEL | 2022年 / 11卷 / 07期

关键词：

Cannabis sativa L; chemovars; secondary metabolites; trichomes; residual by-products; EXTRACTION; PRODUCT; PLANT;

D O I：

10.3390/plants11070891

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

New hemp (Cannabis sativa L.) strains developed by crossbreeding selected varieties represent a novel research topic worthy of attention and investigation. This study focused on the phytochemical characterization of nine hemp commercial cultivars. Hydrodistillation was performed in order to collect the essential oils (EO), and also the residual water and deterpenated biomass. The volatile fraction was analyzed by GC-FID, GC-MS, and SPME-GC-MS, revealing three main chemotypes. The polyphenolic profile was studied in the residual water and deterpenated biomass by spectrophotometric assays, and HPLC-DAD-MSn and H-1-NMR analyses. The latter were employed for quali-quantitative determination of cannabinoids in the deterpenated material in comparison with the one not subjected to hydrodistillation. In addition, the glandular and non-glandular indumentum of the nine commercial varieties was studied by means of light microscopy and scanning electron microscopy in the attempt to find a possible correlation with the phytochemical and morphological traits. The EO and residual water were found to be rich in monoterpene and sesquiterpene hydrocarbons, and flavonol glycosides, respectively, while the deterpenated material was found to be a source of neutral cannabinoids. The micromorphological survey allowed us to partly associate the phytochemistry of these varieties with the hair morphotypes. This research sheds light on the valorization of different products from the hydrodistillation of hemp varieties, namely, essential oil, residual water, and deterpenated biomass, which proved to be worthy of exploitation in industrial and health applications.

引用

页数：32

共 67 条

[1] A comparative study of seed yield and oil composition of four cultivars of Hemp (Cannabis sativa L.) grown from three regions in northern Iran [J].