Ganoderma lucidum polysaccharide modulates gut microbiota and immune cell function to inhibit inflammation and tumorigenesis in colon

被引：355

作者：

Guo, Cuiling ^{[1
]}

Guo, Dandan ^{[1
]}

Fang, Liu ^{[1
]}

Sang, Tingting ^{[1
]}

Wu, Jianjun ^{[1
]}

Guo, Chengjie ^{[1
]}

Wang, Yujie ^{[1
]}

Wang, Ying ^{[1
]}

Chen, Chaojie ^{[1
]}

Chen, Jiajun ^{[1
]}

Chen, Rong ^{[1
]}

Wang, Xingya ^{[1
]}

机构：

[1] Zhejiang Chinese Med Univ, Coll Pharmaceut Sci, 260 Baichuan Rd, Hangzhou 311400, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2021年 / 267卷

基金：

中国国家自然科学基金;

关键词：

Ganoderma lucidum polysaccharide; Gut microbiota; Colitis-associated cancer; Inflammation; Macrophage; LPS; COLORECTAL-CANCER; INDUCED COLITIS; DIETARY FIBER; MICE; CARCINOGENESIS; DISEASE; MAPK; DIFFERENTIATION; COLONIZATION; SUPPRESSION;

D O I：

10.1016/j.carbpol.2021.118231

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study investigated the effects of water-soluble polysaccharide extracted from the sporoderm-removed spores of Ganoderma lucidum (GLP) against AOM/DSS-induced inflammation, tumorigenesis, and gut microbiota modification, which has never been reported before. Our data revealed that GLP (200 and 300 mg/kg) decreased AOM/DSS-induced colitis and tumorigenesis, manifested by significantly reduced disease activity index score, and total number and size of tumors. Furthermore, GLP ameliorated AOM/DSS-induced microbiota dysbiosis, increased short-chain fatty acid production, and alleviated endotoxemia by inhibiting TLR4/MyD88/NF-kappa B signaling. Besides, GLP profoundly improved gut barrier function as evidenced by increased numbers of goblet cells, MUC2 secretion, and tight junction protein expressions. GLP treatment inhibited macrophage infiltration and downregulated IL-1 beta, iNOS, and COX-2 expressions. Additionally, GLP inhibited lipopolysaccharides (LPS)induced inflammation markers and MAPK (JNK and ERK) activation in macrophage RAW264.7, intestinal HT-29, and NCM460 cells. In conclusion, these results indicate that GLP is a promising prebiotic for the treatment of colorectal cancer.

引用

页数：19

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