A 1H/13C inverse 2D method for the analysis of the polyamines putrescine, spermidine and spermine in cell extracts and biofluids

被引：0

作者：

Willker, W ^{[1
]}

Flogel, U ^{[1
]}

Leibfritz, D ^{[1
]}

机构：

[1] Univ NW2, Inst Organ Chem, D-28334 Bremen, Germany

来源：

NMR IN BIOMEDICINE | 1998年 / 11卷 / 02期

关键词：

polyamines; F98 glioma cells; selective HSQC; HSQC-TOCSY; U-C-13]glucose; U-C-13]glutamate; osmotic stress;

D O I：

10.1002/(SICI)1099-1492(199804)11:2<47::AID-NBM500>3.0.CO;2-G

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The polyamines putrescine, spermidine and spermine are involved in the regulation of various metabolic processes. It is therefore desirable to detect and quantify the polyamines with NMR. We present the proton and carbon assignments for all polyamine signals obtained from PCA extracts of F98 glioma cells with high resolution using a semi-selective HSQC 2D-experiment. The biosynthesis of the polyamines in cell culture was examined using the labeled substrates [U-C-13]glucose and [U-C-13]glutamate. In such studies the high resolution of the semi-selective HSQC experiment at very high magnetic fields (14-19 T) allows the analysis of carbon-carbon couplings, and isotopomer patterns. The different effects of osmotic stress on the concentrations of polyamines and amino acids are also reported. (C) 1998 John Wiley & Sons, Ltd.

引用

页码：47 / 54

页数：8

共 18 条

[1] Adaptation of cellular metabolism to anisosmotic conditions in a glial cell line, as assessed by C-13-NMR spectroscopy [J].