Analysis of antigen-presenting functionality of cultured rat hepatic stellate cells and transdifferentiated myofibroblasts

被引：29

作者：

Bomble, Michael ^{[1
]}

Tacke, Frank ^{[2
]}

Rink, Lothar ^{[3
]}

Kovalenko, Evgenia ^{[1
]}

Weiskirchen, Ralf ^{[1
]}

机构：

[1] RWTH Univ Hosp, Inst Clin Chem & Pathobiochem, D-52074 Aachen, Germany

[2] RWTH Univ Hosp, Dept Med 3, D-52074 Aachen, Germany

[3] RWTH Univ Hosp, Inst Immunol, Fac Med, D-52074 Aachen, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 396卷 / 02期

关键词：

Antigen presentation; Hepatic stellate cell; Myofibroblast; Transdifferentiation; Co-culture systems; T-cell response; Immune regulation; Interferon-gamma; T-CELLS; ENDOTHELIAL-CELLS; EXPRESSION; CYTOKINES; RESPONSES; FIBROSIS; RECEPTOR; MICE; MHC;

D O I：

10.1016/j.bbrc.2010.04.094

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we demonstrate that hepatic stellate cells (HSC) isolated from Lewis rats have in vitro antigen-presentation cell (APC) functionality and are able to process and present exogenous antigens. We show activation of a major histocompatibility complex II (RT1BI)-restricted T-cell hybridoma specific for guinea pig myelin basic protein (gpMBP) after coculture with HSC. During transdifferentiation of HSC into myofibroblasts (MFB) the APC function was markedly decreased but restorable by addition of interferon-gamma (IFN-gamma). Based on our findings we conclude that HSC play a key role in hepatic immune function and that IFN-gamma treatment might mediate its beneficial therapeutic effects via activation of APC function in MFB. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：342 / 347

页数：6

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