Fasciola gigantica-Derived Excretory-Secretory Products Alter the Expression of mRNAs, miRNAs, lncRNAs, and circRNAs Involved in the Immune Response and Metabolism in Goat Peripheral Blood Mononuclear Cells

被引：6
作者：
Wang, Sha-Sha [1 ,2 ]
Chen, Dan [3 ]
He, Jun-Jun [1 ]
Zheng, Wen-Bin [4 ]
Tian, Ai-Ling [1 ]
Zhao, Guang-Hui [2 ]
Elsheikha, Hany M. [5 ]
Zhu, Xing-Quan [1 ,4 ,6 ]
机构：
[1] Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou Vet Res Inst, Lanzhou, Peoples R China
[2] Northwest A&F Univ, Coll Vet Med, Yangling, Shaanxi, Peoples R China
[3] Fudan Univ, Sch Sci, Shanghai, Peoples R China
[4] Shanxi Agr Univ, Coll Vet Med, Taigu, Peoples R China
[5] Univ Nottingham, Fac Med & Hlth Sci, Sch Vet Med & Sci, Loughborough, Leics, England
[6] Yunnan Agr Univ, Coll Vet Med, Key Lab Vet Publ Hlth Higher Educ Yunnan Prov, Kunming, Yunnan, Peoples R China
来源：
FRONTIERS IN IMMUNOLOGY | 2021年 / 12卷
关键词：
Fasciola gigantica; peripheral blood mononuclear cells; RNA-seq; excretory-secretory products; long noncoding RNA; ALTERNATIVE ACTIVATION; APOPTOSIS; HEPATICA; GENES; INFECTION; EFFICIENT;
D O I：
10.3389/fimmu.2021.653755
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Fasciola gigantica produces excretory-secretory products (ESPs) with immune-modulating effects to promote its own survival. In this study, we performed RNA-seq to gain a comprehensive global understanding of changes in the expression of mRNAs, miRNAs, lncRNAs, and circRNAs in goat peripheral blood mononuclear cells (PBMCs) treated with F. gigantica ESPs. A total of 1,544 differently expressed mRNAs (790 upregulated and 754 downregulated genes), 30 differently expressed miRNAs (24 upregulated and 6 downregulated genes), 136 differently expressed circRNAs (83 upregulated and 53 downregulated genes), and 1,194 differently expressed lncRNAs (215 upregulated and 979 downregulated genes) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that F. gigantica ESPs altered the expression of genes associated with the host immune response, receptor signaling, disease and metabolism. Results from RNA-seq were validated by qRT-PCR. These findings provide an important resource for future investigation of the role of mRNAs and non-coding RNAs in mediating the immune-modulating effects of F. gigantica ESPs.
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页数：14
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