Introducing genetically encoded aldehydes into proteins

被引：342
作者：
Carrico, Isaac S.
Carlson, Brian L.
Bertozzi, Carolyn R.
机构：
[1] Univ Calif Berkeley, Dept Chem, Howard Hughes Med Inst, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Howard Hughes Med Inst, Berkeley, CA 94720 USA
来源：
NATURE CHEMICAL BIOLOGY | 2007年 / 3卷 / 06期
关键词：
D O I：
10.1038/nchembio878
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.
引用
收藏
页码：321 / 322
页数：2
相关论文
共 15 条
[11]   The human SUMF1 gene, required for posttranslational sulfatase modification, defines a new gene family which is conserved from pro- to eukaryotes [J].
Landgrebe, J ;
Dierks, T ;
Schmidt, B ;
von Figura, K .
GENE, 2003, 316 :47-56
[12]   Non-canonical amino acids in protein engineering [J].
Link, AJ ;
Mock, ML ;
Tirrell, DA .
CURRENT OPINION IN BIOTECHNOLOGY, 2003, 14 (06) :603-609
[13]   A NOVEL AMINO-ACID MODIFICATION IN SULFATASES THAT IS DEFECTIVE IN MULTIPLE SULFATASE DEFICIENCY [J].
SCHMIDT, B ;
SELMER, T ;
INGENDOH, A ;
VONFIGURA, K .
CELL, 1995, 82 (02) :271-278
[14]   Expanding the genetic code [J].
Wang, Lei ;
Xie, Jianming ;
Schultz, Peter G. .
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2006, 35 :225-249
[15]   Labeling proteins with small molecules by site-specific posttranslational modification [J].
Yin, J ;
Liu, F ;
Li, XH ;
Walsh, CT .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (25) :7754-7755
12