Introducing genetically encoded aldehydes into proteins

被引：342
作者：
Carrico, Isaac S.
Carlson, Brian L.
Bertozzi, Carolyn R.
机构：
[1] Univ Calif Berkeley, Dept Chem, Howard Hughes Med Inst, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Howard Hughes Med Inst, Berkeley, CA 94720 USA
来源：
NATURE CHEMICAL BIOLOGY | 2007年 / 3卷 / 06期
关键词：
D O I：
10.1038/nchembio878
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.
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收藏
页码：321 / 322
页数：2
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