Introducing genetically encoded aldehydes into proteins

被引:336
作者
Carrico, Isaac S.
Carlson, Brian L.
Bertozzi, Carolyn R.
机构
[1] Univ Calif Berkeley, Dept Chem, Howard Hughes Med Inst, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Howard Hughes Med Inst, Berkeley, CA 94720 USA
关键词
D O I
10.1038/nchembio878
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.
引用
收藏
页码:321 / 322
页数:2
相关论文
共 15 条
  • [1] Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase
    Chen, I
    Howarth, M
    Lin, WY
    Ting, AY
    [J]. NATURE METHODS, 2005, 2 (02) : 99 - 104
  • [2] In vivo reporter labeling of proteins via metabolic delivery of coenzyme A analogues
    Clarke, KM
    Mercer, AC
    La Clair, JJ
    Burkart, MD
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (32) : 11234 - 11235
  • [3] The multiple sulfatase deficiency gene encodes an essential and limiting factor for the activity of sulfatases
    Cosma, MP
    Pepe, S
    Annunziata, I
    Newbold, RF
    Grompe, M
    Parenti, G
    Ballabio, A
    [J]. CELL, 2003, 113 (04) : 445 - 456
  • [4] Posttranslational formation of formylglycine in prokaryotic sulfatases by modification of either cysteine or serine
    Dierks, T
    Miech, C
    Hummerjohann, J
    Schmidt, B
    Kertesz, MA
    von Figura, K
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (40) : 25560 - 25564
  • [5] Sequence determinants directing conversion of cysteine to formylglycine in eukaryotic sulfatases
    Dierks, T
    Lecca, MR
    Schlotterhose, P
    Schmidt, B
    von Figura, K
    [J]. EMBO JOURNAL, 1999, 18 (08) : 2084 - 2091
  • [6] Multiple sulfatase deficiency is caused by mutations in the gene encoding the human Cα-formylglycine generating enzyme
    Dierks, T
    Schmidt, B
    Borissenko, LV
    Peng, JH
    Preusser, A
    Mariappan, M
    von Figura, K
    [J]. CELL, 2003, 113 (04) : 435 - 444
  • [7] Specific labeling of cell surface proteins with chemically diverse compounds
    George, N
    Pick, H
    Vogel, H
    Johnsson, N
    Johnsson, K
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (29) : 8896 - 8897
  • [8] N-terminal protein modification through a biomimetic transamination reaction
    Gilmore, Joshua M.
    Scheck, Rebecca A.
    Esser-Kahn, Aaron P.
    Joshi, Neel S.
    Francis, Matthew B.
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (32) : 5307 - 5311
  • [9] Specific covalent labeling of recombinant protein molecules inside live cells
    Griffin, BA
    Adams, SR
    Tsien, RY
    [J]. SCIENCE, 1998, 281 (5374) : 269 - 272
  • [10] Effect of pegylation on pharmaceuticals
    Harris, JM
    Chess, RB
    [J]. NATURE REVIEWS DRUG DISCOVERY, 2003, 2 (03) : 214 - 221