Optimised green synthesis of copper oxide nanoparticles and their antifungal activity

Copper oxide (CuO) nanoparticles were synthesised from Ginkgo biloba L. leaf extract and several parameters that affected their formation were adjusted. The optimal synthesis system was ascertained as 10 ml leaf filtrate, 1 mM copper sulfate, and 80 degrees C. The scanning electron microscope image showed that the synthesised CuO nanoparticles were shaped like a short rod. The antifungal effect of CuO nanoparticles against Bipolaris maydis was operated through agar well diffusion, mycelial growth, spore germination, and detached inoculation. At the concentration of 200 mu g/ml, the suppression rate was up to 62.78%, and the spore germination was totally inhibited irrespective of suspension in a liquid or on detached maize leaf. The results are of great significance for comprehensive control of plant diseases, and it also provides a novel antimicrobial to assist or substitute chemical pesticides.