Mechanism of eIF6-mediated Inhibition of Ribosomal Subunit Joining

被引：99

作者：

Gartmann, Marco ^{[1
,2
]}

Blau, Michael ^{[5
]}

Armache, Jean-Paul ^{[1
,2
]}

Mielke, Thorsten ^{[3
,4
]}

Topf, Maya ^{[6
]}

Beckmann, Roland ^{[1
,2
]}

机构：

[1] Univ Munich, Dept Biochem, Gene Ctr, D-81377 Munich, Germany

[2] Univ Munich, Ctr Integrated Prot Sci CIPSM, D-81377 Munich, Germany

[3] Max Planck Inst Mol Genet, UltraStrukturNetzwerk, D-14195 Berlin, Germany

[4] Charite, Inst Med Phys & Biophys, D-10098 Berlin, Germany

[5] Humboldt Univ, Inst Biochem Charite, D-10117 Berlin, Germany

[6] Univ London Birkbeck Coll, Inst Struct & Mol Biol, Sch Crystallog, London WC1E 7HX, England

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2010年 / 285卷 / 20期

关键词：

SACCHAROMYCES-CEREVISIAE HOMOLOG; EUKARYOTIC INITIATION FACTOR-6; ELECTRON-MICROSCOPY; 80S RIBOSOME; PROTEIN; TRANSLATION; YEAST; VISUALIZATION; TRANSLOCATION; REFINEMENT;

D O I：

10.1074/jbc.C109.096057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During the process of ribosomal assembly, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from Saccharomyces cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in Escherichia coli). Based on our structural data, we propose that the mechanism of the anti-association activity of eIF6 is based on steric hindrance of intersubunit bridge formation around the dynamic bridge B6.

引用

页码：14848 / 14851

页数：4

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