IFN-τ Alleviates Lipopolysaccharide-Induced Inflammation by Suppressing NF-κB and MAPKs Pathway Activation in Mice

被引：30

作者：

Wu, Haichong ^{[1
]}

Zhao, Gan ^{[1
]}

Jiang, Kangfeng ^{[1
]}

Chen, Xiuying ^{[1
]}

Rui, Guangze ^{[1
]}

Qiu, Changwei ^{[1
]}

Guo, Mengyao ^{[1
]}

Deng, Ganzhen ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Vet Med, Dept Clin Vet Med, Wuhan 430070, Peoples R China

来源：

INFLAMMATION | 2016年 / 39卷 / 03期

基金：

中国国家自然科学基金;

关键词：

IFN-tau; LPS; anti-inflammation; NF-kappa B; MAPKs; ACUTE LUNG INJURY; INDUCED RAW264.7 MACROPHAGES; INTERFERON-TAU; IN-VITRO; TNF-ALPHA; CELLS; RECOGNITION; POLYMORPHISMS; COMPONENTS; INHIBITION;

D O I：

10.1007/s10753-016-0348-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

IFN-tau, which is a type I interferon with low cytotoxicity, is defined as a pregnancy recognition signal in ruminants. Type I interferons have been used as anti-inflammatory agents, but their side effects limit their clinical application. The present study aimed to determine the anti-inflammatory effects of IFN-tau in a lipopolysaccharide-stimulated acute lung injury (ALI) model and in RAW264.7 cells and to confirm the mechanism of action involved. The methods used included histopathology, measuring the lung wet/dry ratio, determining the myeloperoxidase activity, ELISA, qPCR, and western blot. The results revealed that IFN-tau greatly ameliorated the infiltration of inflammatory cells and the expression of TNF-alpha, IL-1 beta, and IL-6. Further analysis revealed that IFN-tau down-regulated the expression of TLR-2 and TLR-4 mRNA and the activity of the NF-kappa B and MAPK pathways both in a lipopolysaccharide-induced ALI model and in RAW264.7 cells. The results demonstrated that IFN-tau suppressed the levels of pro-inflammatory cytokines by inhibiting the phosphorylation of the NF-kappa B and MAPK pathways. Thus, IFN-tau may be an optimal target for the treatment of inflammatory diseases.

引用

页码：1141 / 1150

页数：10