Elucidation of two O-chain structures from the lipopolysaccharide fraction of Agrobacterium tumefaciens F/1

被引：11

作者：

De Castro, C ^{[1
]}

Carannante, A ^{[1
]}

Lanzetta, R ^{[1
]}

Nunziata, R ^{[1
]}

Piscopo, V ^{[1
]}

Parrilli, M ^{[1
]}

机构：

[1] Univ Naples Federico II, Dipartimento Chim Organ & Biochim, I-80126 Naples, Italy

来源：

CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 14期

关键词：

Agrobacterium tumefaciens; O-chain; LPS; structure;

D O I：

10.1016/j.carres.2004.07.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Agrobacterilan tumefaciens F/1 produces two different O-chains, both are constituted of rhamnose and glucosamine: the less abundant has a linear disaccharidic repeating unit 3)-alpha-L-Rhap-(1 --> 3)-beta-D-GlcpNAc-(1 --> and the second one 4)-alpha-I.-Rhap(1 --> 3)-beta-D-GlcpNAc-(1 -->. The two intact antigenic moieties were studied in mixture by 2D NMR. Additional supporting data were obtained by periodate degradation, the major component was cleaved selectively, leading to a glucosamine glycoside, whereas the minor one was recovered unaffected. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：2451 / 2455

页数：5

共 15 条

[1] The O-specific chain structure of the major component from the lipopolysaccharide fraction of Halomonas magadii strain 21 MI (NCIMB 13595) [J].

de Castro, C ;

Molinaro, A ;

Nunziata, R ;

Grant, W ;

Wallace, A ;

Parrilli, M .

CARBOHYDRATE RESEARCH, 2003, 338 (06) :567-570

[2] Elucidation of the O-chain structure from the lipopolysaccharide of Agrobacterium tumefaciens strain C58 [J].

De Castro, C ;

Bedini, E ;

Nunziata, R ;

Rinaldi, R ;

Mangoni, L ;

Parrilli, M .

CARBOHYDRATE RESEARCH, 2003, 338 (18) :1891-1894

[3] Structural determination of the O-chain polysaccharide from Agrobacterium tumefaciens, strain DSM 30205 [J].

De Castro, C ;

De Castro, O ;

Molinaro, A ;

Parrilli, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2002, 269 (12) :2885-2888

[4]

FURN S, 1985, CARBOHYD RES, V139, P293

[5] A NEW METHOD FOR EXTRACTION OF R-LIPOPOLYSACCHARIDES [J].

GALANOS, C ;

LUDERITZ, O ;

WESTPHAL, O .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 9 (02) :245-&

[6]

HAY G. W., 1965, METHOD CARBOHYD CHEM, V5, P357

[7] NUMERICAL TAXONOMIC ANALYSIS OF AGROBACTERIUM [J].

KERSTERS, K ;

DELEY, J ;

SNEATH, PHA ;

SACKIN, M .

JOURNAL OF GENERAL MICROBIOLOGY, 1973, 78 (OCT) :227-239

[8] SENSITIVE SILVER-STAINING DETECTION OF BACTERIAL LIPOPOLYSACCHARIDES IN POLYACRYLAMIDE GELS [J].

KITTELBERGER, R ;

HILBINK, F .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1993, 26 (01) :81-86

[9]

KJAER M, 1994, METHODS ENZYMOL, V239, P288

[10]

LAEMMLI UK, 1970, NATURE, V97, P620

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