miR-139-5p Represses BMSC Osteogenesis via Targeting Wnt/β-Catenin Signaling Pathway

被引:86
作者
Long, Haitao [1 ]
Sun, Buhua [1 ]
Cheng, Liang [1 ]
Zhao, Shushan [1 ]
Zhu, Yong [1 ]
Zhao, Ruibo [1 ]
Zhu, Jianxi [1 ]
机构
[1] Cent S Univ, Xiangya Hosp, Dept Orthoped, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
miR-139-5p; hBMSCs; osteogenesis; Wnt/beta-catenin; FZD4; CTNNB1; BETA-CATENIN; OSTEOBLAST DIFFERENTIATION; BONE REGENERATION; EXPANDING ROLE; CANCER CELLS; BSP; OSTEOPOROSIS; ACTIVATION; EXPRESSION; PROMOTE;
D O I
10.1089/dna.2017.3657
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Osteogenesis of mesenchymal stem cells (MSCs) has played a necessary role in the repair of bone. According to some reports, microRNAs participate in different physiological activity of the cells, including cell differentiation. This study investigated the function that miR-139-5p plays in the osteogenic differentiation of human bone marrow MSCs (hBMSCs). In addition to miR-139-5p, the effects of alkaline phosphatase (ALP), a membrane-bound metalloenzyme that is considered an early osteogenic differentiation marker, have also been investigated. Calcium-rich deposit (mineralization) is also a typical osteogenic differentiation marker that could be visualized by alizarin red S (ARS) staining. Inhibiting miR-139-5p notably promotes the hBMSC osteoblast differentiation, which, however, will be reduced by overexpressed miR-139-5p. This result has been made based on the alternations of ALP activity, ARS staining, as well as expression of osteogenic genes, including runt-related gene-2 (Runx2), collagen I (Col-1), and osteocalcin (OCN). miR-139-5p exerts its role in BMSC osteogenesis most probably through the Wnt/beta-catenin pathway, by direct targeting CTNNB1 and frizzled 4 (FZD4), essential factors of Wnt/beta-catenin pathway. In conclusion, according to the present study, inhibiting miR-139-5p could be a promising strategy in hBMSC osteogenesis.
引用
收藏
页码:715 / 724
页数:10
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