Curcumin alleviates ethanol-induced hepatocytes oxidative damage involving heme oxygenase-1 induction

被引：110

作者：

Bao, Wei ^{[1
,2
]}

Li, Ke ^{[1
,2
]}

Rong, Shuang ^{[1
,2
]}

Yao, Ping ^{[1
,2
]}

Hao, Liping ^{[1
,2
]}

Ying, Chenjiang ^{[1
,2
]}

Zhang, Xiping ^{[1
,2
]}

Nussler, Andreas ^{[3
]}

Liu, Liegang ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Dept Nutr & Food Hyg, Wuhan 430030, Peoples R China

[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Minist Educ,Key Lab Environm & Hlth, Wuhan 430030, Peoples R China

[3] Tech Univ Munich, Dept Traumatol, D-81675 Munich, Germany

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2010年 / 128卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Curcumin; Heme oxygenase-1; Oxidative stress; Alcoholic liver disease; PROTECTS HUMAN HEPATOCYTES; IN-VITRO; ALCOHOL; STRESS; INJURY; WARM; RATS;

D O I：

10.1016/j.jep.2010.01.029

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance. Curcumin is the main bioactive constituent derived from the rhizome of turmeric (Curcuma longa Linn), which has been used traditionally as hepatoprotective agents in ayurvedic and traditional Chinese medicine for centuries. Aim of the study The present study was carried out to demonstrate the potential protective effect of curcumin pretreatment against ethanol-induced hepatocytes oxidative damage, with emphasis on heme oxygenase-1 (HO-1) Induction Materials and methods. Rat primary hepatocytes were isolated and treated with ethanol (100 mM) and diverse closes of curcumin (0-50 mu M), which was pretreated at various time points (0-5 h) before ethanol administration Hepatic enzyme releases in the culture medium and redox status including HO-1 enzyme activity were detected Results. Ethanol exposure resulted in a sustained malondialdehyde (MDA) elevation, glutathione (GSH) depletion and evident release of cellular lactate dehydrogenase (LDH) and aspartate aminotransferase (AST), which was significantly ameliorated by curcumin pretreatment In addition, dose- and time-dependent induction of HO-1 was involved in such hepatoprotective effects by curcumin Conclusions. Curcumin exerts hepatoprotective properties against ethanol involving HO-1 induction, which provide new insights into the pharmacological targets of curcumin in the prevention of alcoholic liver disease. (C) 2010 Elsevier Ireland Ltd All rights reserved

引用

页码：549 / 553

页数：5

共 22 条

[1]

Aggarwal BB, 2007, ADV EXP MED BIOL, V595, P1

[2]

Akerboom T P, 1981, Methods Enzymol, V77, P373

[3] How many transcription factors does it take to turn on the heme oxygenase-1 gene? [J].

Alam, Jawed ;

Cook, Julia L. .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2007, 36 (02) :166-174

[4] Alcohol, oxidative stress and free radical damage [J].

Albano, Emanuele .

PROCEEDINGS OF THE NUTRITION SOCIETY, 2006, 65 (03) :278-290

[5]

Buege J A, 1978, Methods Enzymol, V52, P302

[6]

Clark R, 1997, VET ECON, V38, P41

[7] Molecular understanding and modern application of traditional medicines: Triumphs and trials [J].

Corson, Timothy W. ;

Crews, Craig M. .

CELL, 2007, 130 (05) :769-774

[8] Curcumin attenuates dimethylnitrosamine-induced liver injury in rats through Nrf2-mediated induction of heme oxygenase-1 [J].

Farombi, E. Olatunde ;

Shrotriya, Sangeeta ;

Na, Hye-Kyung ;

Kim, Sung-Hoon ;

Surh, Young-Joon .

FOOD AND CHEMICAL TOXICOLOGY, 2008, 46 (04) :1279-1287

[9]

Farombi EO, 2006, J BIOCHEM MOL BIOL, V39, P479

[10]

Koch Osvaldo R., 2004, Molecular Aspects of Medicine, V25, P191, DOI 10.1016/j.mam.2004.02.019

← 1 2 3 →